Human amniotic membrane (HAM) was immersed in diluted honey (0.02% & 0.3%) for 24 hours. After 24h, HAM (1cm2) was immersed and incubated in PBS (in 12 multi well plate). 1ml of PBS was collected at pre-determined time (1h, 3h, 12h, 24h & 72h) to observe the release of honey from HAM over time. 1ml of PBS was replenished at every sampling time.