Please guide me in a simple way how to determine correlation of phenolic content with antioxidants please..
If the correltation of the two tells you anything, you can do the calculations in Microsoft Excel by using a simple formula as follows:
Dear Idrees M.,
your question is ill formulated. Probably you asked about " correlation of phenolic content " with antioxidant activity (how fast they are) or with TAC (Total Antioxidant Capacity, the amount of antioxidants in 1 g of your sample). I could predict that TAC would be linearly dependent on phenolic content , and the activity would be weakly dependent on phenolic content. While, it might significantly depend on the nature of "phenolics"
Dear Tadesse Fikre Teferra , I agree with you but you did not mention what two "parameters" should be used.
Dear Mariel Gullian Klanian , I recommended your very professional answer. I would post the same with some minor variations.
The question is not HOW but WHY, specially considering that you are actually "measuring" your polyphenols using an antioxidant assay (Folin-Ciocalteu measures reducing capacity). So of course you'll find high correlations, as has been reported thousands of times. But they are not relevant as you have not actually measured the polyphenols, this requires HPLC, probably also solvolysis, and a good kowledge of extraction methods.
You have to measure polyphenolic contents of samples by
Folin-Ciocalteu method and their antioxidant activity by ABTS and DPPH methods.
@Maitree-Suttajit: no, this is a tautology, because the Folin Ciocalteu is NOT speciifc for polyphenols, it is an antioxidant assay too.
For more the specificity, I agree with you. thanks. However, the question asked about the relationship or "Correlation of phenolic content with antioxidants? "
I've already commented that this question is ill formulated.
It was asked " How to determine Correlation ". In order to find a correlation between two values you have to apply "statistical analysis." That's all!
Yurii V Geletii Since we are interested in causation, simply finding a correlation is NOT sufficient. We must establish and identify the causal mechanism, and show that it is causation, and not simply correlation. Is this correct?
The antioxidant beneficial effects of polyphenols on our body or organs are dependent on their bioavailability.
Olaniyi Amos Fawole FC assay DOES NOT measure the total antioxidant content! It does not measure the total phenol content. This work has been cited more than 13000 times
Article Analysis of Total Phenols and Other Oxidation Substrates and...
FC measures the total number of electrons from a sample to reduce Mo(VI) to Mo(V) under conditions of the assay. A calibration, e.g. using gallic acid, gives a relative number of electrons compared with gallic acid. This is well explained in the ref , Which I provided earlier.
Maybe let's start with a graphical illustration of the data. Moreover, which phenolic compounds, because most of them, have antioxidant properties. So it is as if we wanted to find a correlation between antioxidants and antioxidants. Well. As I can guess, it is enough to use ANOVA.
Dear Mirosław Grzesik , you are absolutely right that "So it is as if we wanted to find a correlation between antioxidants and antioxidants." FC can be considered as an antioxidant assay for a certain family of chemical compounds, such as phenolics. For a correlation you need two numbers. Thus, a second antioxidant assay should be used for the same family of compounds. I guess that this has been already done and published.
The questioner should better specify what the question is about. Why guess? But that sounds like a typical food chemistry issue to me.
First find the antioxidant capacity for a specific concentration of sample
then find the phenolics for the same concentration of sample
then find the correlation between antioxidant capacity and phenolics
Mirosław Grzesik is absolutely right that "So it is as if we wanted to find a correlation between antioxidants and antioxidants."
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