Does anyone have a simplest protocol for the extraction of carbamate (carbofuran) pesticides from liquid and soil samples?

In order to study the microbial degradation of carbofuran. I need a simple and less time taking easy extraction protocol for carbofuran. Thanks

10 November 2019 5,920 4 View

Instead of osmium tetroxide is there any other substance that can be used as a secondary fixative for electron microscopy of bacterial cell?

08 September 2016 4,153 5 View

What is the best method to purify a bacterial protease enzyme?

I am currently working on a protease enzyme from bacteria doing casein hydrolysis. Can anyone please give me a protocol how to purify it?

03 April 2015 5,565 5 View

What is the effect of tannery effluent on soil?

Tannery effluent contains a huge amount of heavy metals and chemicals.

03 April 2012 650 1 View

Please give me protocol for the study of heavy metal tolerance and antibiotic resistance property in bacteria isolated from tannery effluent.

Please anyone provide me some information

01 February 2012 5,838 2 View

How micro-organisms show resistance to heavy metals?

As heavy metal pollution is a major threat to the environment.

01 February 2012 9,251 3 View

Why Trivalent chromium start precipitating above PH 5 ? Moreover discuss difference between adsorption of chromium and precipitation of chromium ?

I am a student of MS Environmental Engineering and started my research phase. I am working on metal removal ( chromium iii ) by using activated carbon prepared from the agricultural feedstock. So...

01 March 2021 5,475 2 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

Passivation of chromium cast Iron in high temperature?

14 February 2021 2,157 3 View

How long does it take to design and validate transition state analogue inhibitors?

Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...

14 February 2021 2,610 1 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

How to determinate of molecular structure of salt into the liquid?

I need to determine the open formula of our complex, presumably consisting of sodium tungstate Na2WO4 and aluminum sulfate Al2 (SO4) 3. Our problem is that with an increase in the concentration...

11 February 2021 5,821 1 View

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Hello! I want to determine the activity of my in house Reverse Transcriptase enzyme. For this, I have used Thermo's Enzcheck Reverse Transcriptase assay kit. I used commercial Thermo RT enzyme to...

08 February 2021 7,285 1 View

How define one baker unit(BU) is the amount of hydrogen peroxide catalase ?

07 February 2021 2,006 1 View

Electron density difference maps(Fo-Fc): what is the meaning of negative densities (red blobs)?

Dear community, As far as I understand, negative density features point at stuff that is in the model, but not supported by experimental data. However, in many cases (e.g. attached image), when...

06 February 2021 2,847 2 View

Does DNA A-tailing survive after agarose gel electrophoresis and purification?

Hi everyone, I need to clone in pGEM-T vector the result of amplifying a metagenomic library by PCR with a high fidelity polymerase but I only want to clone the fragments of an specific size...

04 February 2021 6,051 8 View