for mice, get blood from IVC using a syringe, the circulation blood volume could be estimated.

Hi, Prof. Zhang, thanks for your answer. Can you give me a reference or a detailed explanation? I am not sure I can get your idea. 

Dear Zhang

To evaluate blood volume, you first need to calculate plasma volume using Evans blue, which is injected into the circulating blood. Subsequently, the sample of blood is then drawn and the exact quantity of dye is measured. Then you need to take into account the hematocrit value and calculate the final volume

for reference about Evans blue dilution method

see : Clin Lab Haematol. 1995 Jun;17(2):189-94.

Best Regards

Peter

Dear Kubisz,

Thanks for your information. I checked the literature and got the information.

Best regards