How to determine anti inflammatory in extract by spectrophotometer?
Dear Siddharth,
You may use Nitric Oxide assay and related procedures mentioned in the attached paper.
Best wishes,
P
http://webcache.googleusercontent.com/search?q=cache:rNArXKqqNDoJ:bmccomplementalternmed.biomedcentral.com/articles/10.1186/1472-6882-12-173+&cd=1&hl=en&ct=clnk&gl=in
Dear Prakash Pradhan
please can you download or send me this paper it doesnot respond i need it
thanks
hi prakash,
be specific. however, this might help
good luck
mathipi
Try LPS induced inflammation in RAW cell line. Do cell viability assay (MTT).
Sir we dont work on cell line.Is there any other method using spectrophotometer?
Assessment of invitro antiinflammatory activity Inhibition of albumin denaturation???????
Try nitric oxide scavenging ability of plant extract.
Just googled it, you will get procedure.
But it is not that reliable, you have to perform cell line study (or) paw edema test in animals.
All the best
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