07 December 2020 6 4K Report

Questions regarding biotechnology and microbiological and bio chemistry DEPARTMENT practically project working my contacts number is 8248413604

More Vithlani Siddharth's questions See All
Cloning of flaviviruses is difficult i have noticed that cells loose their transformation ability after cloning fragments inside vector any advice?

As flaviviruses genome is very frequently prone to mutation hence it is very difficult to clone whole genome inside vector what kind of vector and cells can sustain such amount of cloning...

11 July 2024 4,284 0 View

I am not getting proper colonies while ligating one of fragments in my vector the colonies seems to be normal but in light it seems lysed any reason?

I am working on cloning of a gene fragment inside my vector everything we tried to do correctly like making insert and vector with utmost care but after ligation the morphology of colonies seems...

11 July 2024 9,010 3 View

How can I plot DOS till 20 eV please tell me?

I have made changes in .in1 as well as in .int files but unable to get dos till 20 eV

13 June 2024 9,080 1 View

Why generally we prefer phenol chloroform extraction method for DNA purification as i always face difficulty in getting good concentration why?

phenol chloroform extraction method is used for purification of nucleic acids DNA/RNA

03 April 2024 8,268 0 View

After phenol chloroform extraction the nucleic acid concentration is around 20ng/ul but after loading 1ul/2ul its still not visible why?

phenol chloroform extraction method is used to purify nucleic acids to obtain a good concentration.

03 April 2024 7,244 0 View

Can anyone suggest some specific bacterial cell line for cloning viruses, specifically flaviviruses i have used cell like TOP-10/DH10B/DH5 suggest?

as viruses are notorious for being prone to mutation at high rates its very difficult to clone them though general cloning procedures and cells so in order to solve this problem need specific cell...

03 April 2024 9,031 5 View

Why we have two differnt formulas for the calculation of seebeck coefficient in theoretical and experimental methods?

For the boltaztrap we used the formula for calculating the Seebeck coefficient using a different formula mentioned in doi:10.1016/j.cpc.2006.03.007 and the formula we are using in the experiment...

17 March 2024 2,105 3 View

How much "instant" a photon is?

There are two rooms: Room A and Room B; initially both are dark; contains no light source (of whatsoever). Now, I bought a light source and set it up in the room A, enabling me to see things...

28 February 2024 4,809 10 View

What are R packages for detecting peaks and peaks area for HPLC chromatogram?

I am performing snake venom fractionation using Reverse-phase HPLC. It generates multiple peaks with different area under the curve. Since I'll be analyzing multiple chromatograms at different...

08 November 2023 9,134 0 View

Looking for an opportunity to visit any of the campus in the United States working in the area of biotechnology. Please respond and can you help?

Dear All, Biotechnologists from The area of Biotechnology. Currently I am serving as HoD in Department of Biotechnology, IMSEC, New Delhi NCR. Is there any person who can help me for two or three...

08 November 2023 2,785 4 View

Similar questions and discussions
How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

What researches are there for satisfaction level of the hospital attachment for student nurses ?

I am doing a study on nursing students satisfaction on the hospital attachment whether they are satisfied with the clinical attachment or not. I need more research studies on this

03 August 2024 6,985 2 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

What exactly is it about biotechnology and medical research?

I'm very interested in biotechnology and medical research and I'd like to meet a professional here and talk about it together

28 July 2024 3,798 4 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View

How can I use DFT-D3 correction ( Siesta version 5.0.1)?

Dear Siesta Users,  I installed Siesta 5.0.1 and I want to use Grimme's D3 correction. According to the 5.0.1 manual, I could use a few parameters to include the D3 correction in the...

27 July 2024 5,748 0 View

EDC carbodiimide and NHS easter 's half life among different pH?

It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...

25 July 2024 8,738 0 View

Mechanochemistry: ball to powder mass or ball to powder volume?

I run mechanochemical reactions that involves the use of a large amount of catalyst (1:1 molar ratio to the reactant). When I try to understand the effect of amount of catalyst on the reaction, I...

23 July 2024 9,938 1 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close