i have detected alkaloid, phenol and tannin from my bacteria sample than i did hpltc. as standards were not available i am unable to detect the exact compound from tthe peak. can any one suggest if there is any standard library
Hello, I'm conducting a beta galactosidase staining assay for my fibroblasts using X-Gal. I dissolve X-Gal (stock 50mg/ml prepared in DMSO) in the staining buffer at a final concentration of...
25 March 2024 8,799 3 View
The absence of Ocean Island Basalts (OIBs) in the Archean rock records is commonly attributed to the higher melting degrees associated with mantle plumes. However, this explanation raises...
14 March 2024 2,195 1 View
Mostly bacteria living inside plant roots.
14 March 2024 4,602 0 View
What is the correct way to calculate the BSSE correction for a molecule having 3 fragments? Please see the attached image for details. I know the values are pretty close but still I would love to...
25 October 2023 3,990 1 View
Can I use DIN and PO4 in the given formula : The trophic index (TRIX) used in the present work has been given by the following formulation (Vollenweideret al.1998): TRIX = [log(Chla∙ (D%)O2 ∙ TN ∙...
26 September 2023 432 1 View
Many think that, because of the vastness of the oceans, the continents were underwater throughout the Hadean and much of the Archean periods. It wasn't until the Mesoarchean that continents began...
19 September 2023 6,875 0 View
I am trying to quantify 5mC and 5hmC using Epimark 5mC and 5hmC kit from NEB. I did the end-point PCR after glucosylation and digestion with MspI and HpaII. It didnt generate a a yes or no answer,...
06 September 2023 6,221 0 View
How can the number of carbon quantum dots (CQDs) internalized per cell be calculated using the "IntraCell V2" plugin in ImageJ?
01 September 2023 419 0 View
The EPMA analysis for FeO for native metal (Iron) is coming as 110, 124, 114 (wt%). What could be the possible reason?
20 July 2023 2,583 0 View
I need to calculate oxygen exchange current density using polarization resistance.
07 June 2023 9,237 2 View
These fungal slides are isolated from cultured Bread mold on PDA media and smear is treated with Lacto phenol Blue dye ..
13 July 2024 9,701 1 View
Aa
11 July 2024 4,645 3 View
Hello, all I am currently extracting LPS from E. coli MG1655 and its mutant strains. I followed several different protocols (phenol methods or phenol+diethy ether methods), but I couldn't see...
25 June 2024 7,568 1 View
I have performed GC-MS analysis of Phytochemicals from a medicinal plants and also identified compound using NIST library. After NIST identification, I have a list of compounds. I am constructing...
12 June 2024 235 4 View
fungal inoculum treated with lacto phenol blue and observed under microscope anyone help me to identify and share some information regarding this fungal slide
07 June 2024 9,647 3 View
Hello everyone, I would like to know if any of you ever tried to assess the yeld of RNA extraction with the mirVana™ miRNA Isolation Kit, without phenol from ThermoFisher. I am experincing some...
06 June 2024 5,715 4 View
I have been making hydrogels regularly using 10X DMEM (phenol red indicator), Ultrapure water, 1N NaOH, and 3.41 mg/ml Collagen (suspended with acetic acid). We have used the same calculations for...
29 May 2024 4,655 0 View
The addition of Na2CO3 in TPC estimation creates an alkaline medium for phenols to get unstable, however whenever I add 2 ml of 20% of Na2CO3 to 1 ml of gallic acid extract and 0.5 ml of FC...
14 May 2024 5,478 2 View
I prepared an enrichment media making use of the two samples spoilt Sheabutter and Ghee (differently) and it was cultured on Olive oil agar using pour plate method. The organisms were subcultured...
05 May 2024 5,795 1 View
This is Problem K7 from Chapter "Alkaloids" (Special Topic K), pg 1004.
03 May 2024 8,818 0 View