i have detected alkaloid, phenol and tannin from my bacteria sample than i did hpltc. as standards were not available i am unable to detect the exact compound from tthe peak. can any one suggest if there is any standard library
I tried to fit the frequency vs Dielectric constant and f vs Dielectric Loss graph with HN equation. I got different Tau (relaxation time) values from the fittings of both graphs. What I have to...
12 January 2021 8,467 6 View
In most of the research paper people used both neutral and alkaline comet assay to determine the level of DNA damage either single stranded or double stranded but the exact principle of both the...
02 August 2020 2,193 2 View
In many countries, the children belong to remote villages and do not have facility of computer or Android mobile and internet connectivity. Do you think, the countries will be able provide online...
17 May 2020 2,739 16 View
Using TURBOMOLE, I am studying a mechanism of Heterolytic vs Homolytic cleavage. When the bond breaks, there are three possibilities a) Heterolytic Cleavage (RHF, singlet) b) Homolytic Cleavage...
15 October 2019 4,214 5 View
Need methods for isolation and characterization of bio-active peptides in milk with proper protocols for the same.
12 December 2018 3,818 2 View
I want to obtain the vegetation index map for a date which lies in between the 16 day composite MODIS vegetation indices. Ex- Let's say the MODIS (both terra and aqua) has 16 day composite images...
01 September 2018 9,489 6 View
Logistic regression, SVM, andom forest etc
24 July 2018 1,019 4 View
I have come across a lot of journals with different solutions. But I am not sure which one can be used. Please suggest.
19 July 2018 1,903 1 View
In detail, I performed flexural test of some composite specimen. I got higher flexural strength with high modulus. But after doing some modification in main material, I got enhanced flexural...
15 April 2018 4,826 7 View
I am looking into monocytes and macrophage in murine model (C57Bl/6) of a disease.
22 March 2018 959 2 View
We want to use phenol pH graphic to measure the pH of mediums before and after inhibitor application to cells. We have phenol absorbence values at 460, 560 and 720 nm wavelength. When we measure...
20 February 2021 7,700 3 View
I am currently preparing to isolate polyphenolics (Hydrolysable tannins) from aqueous extract of a plant. I want to set up a small sephadex column to guide the major isolation on a laerge...
18 February 2021 1,455 3 View
I use para nitrophenyl palmitate substrate with tris buffer ph 8... i read many temperature for Yellow color of para nitro phenol production.. 37 and 50 and 40 at water bath ... I confused as i...
04 February 2021 9,100 5 View
Hello everyone, Does anybody know if phenolic plant extracts such as tannins dissolved in water can interact with silicone oil? (although silicone oil and water are immiscible).
03 February 2021 5,656 2 View
I am trying to run a cell viability assay with LN229, but these cells are cultured in DMEM which contain phenol red. Is the MTS assay sensitive to the additional of phenol red in the media? If so,...
03 February 2021 9,478 2 View
Dear Respectable Scientists, I have been trying to extract DNA from above mentioned sources using different CTAB/SDS Buffer Compositions and purifying agents but I am not able to extract DNA from...
12 January 2021 5,899 8 View
07 January 2021 6,527 3 View
I am trying to extract tannin from agricultural produce such as fruits, food, peel, leaf..... I need a reference that it gives me tannin percent of agricultural produce.
05 January 2021 6,695 2 View
Descriptions and simple method of alkaloid extraction
01 January 2021 5,830 6 View
Hi. I'm trying to detect the phenol red in the mixture solution by HPLC. As you know, It is using for ph indicator. The problem is.. After making phenol red solution in 50 ml conical tube, I...
13 December 2020 5,511 4 View