we can detect it with animal studies, or treatmented in human 27-28 defect region open curettage than 28 number must be extracted post op 6 th months so that we can detect near 28 mezial junctional healing with electron microscope ..
The only way is by doing histology. Nevertheless, evidence has demonstrated that areas with long JE are not more prone to periodontal break down than areas with CT attachment. So, clinically it doesn’t matter. As a matter of fact, after periodontal surgery, most likely you will have healing by long JE, even in GTR surgeries you will have the coronal portion healing as a long JE.
I agree with Carra the only way to determine type of attachment is by histology, which could not be conducted ethically on human.
because clinically it could not be differentiated if the attachment is a long junctional or connective tissue attachment which is most probably be a long junctional epithelial attachment after conventional periodontal therapy
If you want a reference: Magnusson et al. A long junctional epithelium - a locus minors resistentiae in plaque infection? Journal of Clinical Periodontology 1983.