How to detect iodine uptake?

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How do you collect the celiac ganglion from mice?

Does anyone know how to / anatomical landmarks to collect the celiac ganglion?

06 December 2023 8,709 0 View

Can you tell me a protocol for the differentiation of mesenchymal stem cells into oligodendrocytes?

Hello everyone, Can you tell me a standardized protocol for the differentiation of mesenchymal stem cells into oligodendrocytes?

14 November 2023 1,546 1 View

Freeman Tukey test: is there an online tool?

Hi, I have data in form of counts (number) and I wanted to run a statistical test to compare the counts between groups to see if there is statistically significant difference between groups. I...

24 October 2023 1,545 3 View

How can I prevent exosomes from clumping together in PBS for NTA after isolation?

I used the ExoSpin kit to isolate EVs from Plasma using 0.22 double filtration and 10KDa Ultrafiltration device and resuspended in PBS (cacl and mg free) and still find that particles are clumping...

13 August 2023 4,773 0 View

Has anyone had problems with the PyroMark Q48 cartridges?

Hi everyone, a few months ago we bought the PyroMark Q48 to replace the old Q96, but we used it very little due to continuous malfunctions of the cartridge with enzyme and substrate. We've already...

23 July 2023 8,854 0 View

What is the optimal concentration of glutamate for patch clamp recordings in mouse brain slices?

What is the optimal concentration of glutamate for patch clamp recordings in mouse brain slices? Can someone provide a study on the dose-response relationship of glutamate in brain slice preparations?

11 July 2023 9,547 1 View

At which step of a staining for FACS do I add Hoechst? Can I use it like DAPI?

at which step of a staining for FACS do I add Hoechst? Can I use it like DAPI?

13 June 2023 7,880 2 View

Is it possible to quantify 16S regions in a sample of WGS data?

Hi, I would like to know if there is any software readily available which allows the identification and quantification for 16S regions in WGS data. Thanks n advance for any advice/help :)

15 May 2023 1,177 0 View

How do you estimate parts of the biofilm being sediments or dead organic material of biomass collected from river stones?

We measured chlorophyll, dry mass and ash-free dry mass and after we calculated autotrophic index (AI). I found in Hauer, F. R., & G. A. Lamberti, 2017. Methods in stream ecology. Elsevier,...

27 March 2023 2,413 1 View

I keep encountering this error when trying the Cast transects in DSAS?

--------------------------- DSAS Error --------------------------- An unexpected error occurred. Please refer to the log file (C:\Users\Deighl\AppData\Roaming\Corona Environmental Consulting\DSAS...

23 January 2023 7,611 0 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 726 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

What is your major research area?

My major research area is clinical research and basic research of thyroid and parathyroid.

26 July 2024 3,639 14 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View

When I am trying to distill triethyl amine while drying it over calcium hydride. How do I design the setup to let hydrogen escape without losing NEt3?

I am new to research. The boiling point of triethyl amine (NEt3) is 89 degrees centigrade. I am skeptical that when I will let the hydrogen escape which is getting generated in situ, I will also...

21 July 2024 7,284 4 View

Does anyone notice the tumor regression impact of Hepa1-6 cells in HCC orthotopic mice model?

To establish an orthotopic HCC model, C57BL/6 mice (male, 6-8 weeks) were orthotopically implanted with 3x106 Hepa1-6 cells (Over-expressed with a human gene) in 20uL Matrigel into the left lobe...

18 July 2024 9,177 2 View

Im trying iodination of an arene, but only getting black precipitates, is there anyone with iodination experience who can advise?

Organic Synthesis. My student has been trying iodination of arenes (our goal is to iodinate p-phenylenediamine) following these literature procedures: 1. Synthesis 2004, No. 11, 1869-1873 using...

16 July 2024 6,630 1 View