How to quantify total protein in crooked western lanes?

I've been using Revert700 total protein stain to detect total protein in my western lanes. While this stain is robust & reversible, I have trouble quantifying in Image Studio Lite (LiCor) and...

05 December 2020 9,014 3 View

How do you treat forced swim test data?

My design is a two factor 3x5 (3 hormone conditions and 5 drug conditions, 15 groups total) between-subjects ANOVA. When rats undergo the 15 minute pre-test habituation forced swim they have not...

11 June 2018 1,558 3 View

How long do I leave a brain in isopentane when flash freezing it for future Western blot analysis?

I have flash frozen brains in the past using isopentane with dry ice in it but I found that some of them cracked. What is the optimal time to leave the brain in the isopentane such that it is...

03 March 2018 2,998 2 View

Dimensions of open field test as a measure of anxiety?

I'm currently testing ketamine as an anti-depressant in female ovariectomized rats. Am curious what size field would be ideal to test the effects of ketamine 24h or 1 week after administration.

28 February 2018 8,395 4 View

How fresh do ruminant fecal samples need to be for next-generation DNA sequencing to determine diet composition?

I need to determine the diet composition and relative proportion of plant species in mule deer diet. What I currently understand is that fecal samples need to be fresh in order to accurately...

26 September 2017 3,848 1 View

SPSS - Standardizing Column Containing Heterogeneous Age Data

Goal: Standardize a column of "Age" data (i.e. into years old / months old) using SPSS / SPSS Syntax. Examples of Cell contents (1 cell is denoted by contents in parentheses): "7" (assume this is...

12 July 2017 6,481 1 View

I am having a problem keeping 3T3-L1 cells alive when differentiating them with troglitazone. Has anyone encountered this?

I grow 3T3-L1 cells for my research. In the differentiation process, I use a medium that contains troglitazone for 2 days. This has worked very well for about 1year, but the past couple of time,...

26 May 2015 8,101 5 View

Can anyone help with a problem regarding lgl1 antibody?

21 August 2014 313 1 View

What are the coolest Machine Learning startups you've seen?

And what do you think the next big thing is in Machine Learning/AI/NLP?

10 July 2013 2,165 12 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

How to remove compound ( Europium complex ) from paraffin oil?

How can I remove my compound from paraffin oil? Mistakenly my compound fell in an oil. Compound = Europium complex

22 February 2021 5,667 2 View

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22 February 2021 9,932 11 View