Collin Pachow

4 Questions 1 Answers 0 Followers

Questions related from Collin Pachow

What could this CD45-, CD11b+ population be?

I'm looking at Helios CyTOF data of virally-infected mouse brains at day 7 post-infection. I've got this large population of CD45-, CD11b+ cells. I'm pretty confident that they're negative for...

05 January 2022 4,702 0 View

How do I interpret the y-axis of a Faith's phylogenetic diversity plot?

Hi all! I'm brand new to microbiome data analysis, so any help here is appreciated. My understanding is that the y-axis is a measure of the sum of the branch lengths for all members in whatever...

19 April 2021 1,351 3 View

Best method to purify viral RNA from cell culture supernatant?

We're trying to decide between the QIAamp Viral RNA Mini Kit, the standard RNeasy Kit, and just using Tri Reagent, which I believe is not a kit. Does anyone have any suggestions or other...

14 September 2020 8,750 5 View

How to detect IgA in paraffin-embedded murine gastric tissue?

I found this protocol: https://www.niehs.nih.gov/research/atniehs/labs/assets/docs/e_j/iga_m_508.pdf However, I'm wondering if anyone has ever attempted this another way (or with the protocol...

27 March 2019 8,393 2 View