How to detect catalase activity in native PAGE electrophoresis?

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Help with GFP fusion protein pulldown?

I am trying to pulldown my GFP fused protein of interest using ChromoTek GFP-Trap® Magnetic Agarose. My protein is not binding to the beads. Expression of the GFP-fusion protein has been...

02 June 2024 8,541 0 View

How can I prepare 3 amino benzoyl chloride from 3 amino benzoic acid and thoinyl chloride and which method and what solvent I have to use ?

How can I prepare 3 amino benzoyl chloride from 3 amino benzoic acid and thoinyl chloride and by which method and what solvent I should use ?

15 February 2024 4,834 0 View

How can I prepare 3 amino benzoyl chloride from 3 amino benzoic acid and thoinyl chloride and which method and what solvent I have to use ?

How can I prepare 3 amino benzoyl chloride from 3 amino benzoic acid and thoinyl chloride by which method and what solvent should I use ?

15 February 2024 5,309 0 View

How to quantify the product from 1H NMR?

I'm getting the formate peak around 8.3 ppm. But the main problem is the quantification of the product. If you could give me some instructions on quantifying the product from the proton NMR, that...

07 February 2024 6,858 2 View

I need Global Car sales data (in units) from 1996 to 2021; especially Philippines and Indonesia. Kindly help, if possible send data or share the link?

I need Global Car sales data (in units) from 1996 to 2021; especially Philippines and Indonesia. Kindly help, if possible send data or share the link. Thank You

14 December 2023 174 0 View

Sadhana journal follows which reference style ?

sadhana journal follows which reference style ?

24 September 2023 4,520 1 View

What is the difference between ambient energy and renewable energy?

What is the difference between ambient energy and renewable energy? Any reference material where I can study these energy sources?

03 September 2023 6,849 7 View

What is the significance of Dead space volume in BET, why and how do we calibrate it and what is the effect of dead space on the experiment?

I'm working on a BET prototype but I'm facing problem in dead space.

28 June 2023 5,609 1 View

How to Stain Poly dT Oligonucleotide?

I have tried with ethidium bromide and stains-all for staining but not getting any band after one day of incubation. I tried with both Native and Non-Native gel.

05 June 2023 377 3 View

Does BET Dosing pressure depend on the adsorbate if both are mesoporous and follow type 4 isotherm?

If we change different type of materials which are in the range of type 4 isotherm and they have similar pore size do we still need to tweak the dosing pressure for both the materials? Even if the...

22 May 2023 9,706 1 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to preform densitometry on SDS-page bands?

I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...

05 August 2024 9,805 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View