Gautam Pandey

3 Questions 18 Answers 0 Followers

Questions related from Gautam Pandey

My 3T3 L1 cells are getting peeled of after giving insulin media post-differentiation medium. What could be the reason ?

Before peeling off cells look very healthy. The media also looks pretty clear. So I think no contamination. I am using passage around 20.

04 April 2016 4,984 4 View

How do I measure glucose consumption by cells in a culture media?

I am culturing 3T3 adipocytes. I want to measure glucose consumption by the cells..One person suggested me to check the glucose in media by normal calorimetric glucose assay (Instead of Glucose...

11 November 2015 5,900 6 View

I am trying to induce lipolysis in 3T3 adipocytes by using Isoproterenol hydrochloride (I6504 Sigma). Why I am not getting any effect?

I have bought Sigma Isoproterenol Hydrochloride (I6504). I have dissolved it in KRBH buffer (pH-7.4) to make it 50 mM stock and stored @ -20. I used 10 micromolar conc. to treat adipocytes for 1-2...

11 November 2015 6,796 13 View