How to design taqmanprobes and primers for methylation analysis on specific promoters and detect by real time PCR?

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In order to run Multinomial Logistic Regression, is it required that the data be in the long format?

I am using unit level data (IHDS round 2) & Stata 17

06 August 2024 5,725 2 View

Has anyone used eric primer for fingerprinting of bacterial isolates?

Hi I am trying to do fingerprinting of rhizobial isolates with the help of eric primer..I prepared a master mix..10x buffer-2.5ul dNTP(10mM) 2ul MgCl2(50mM) 1ul Taq(5U) 0.2 Template 3ul and Eric R...

14 June 2024 981 0 View

I perform battery thermal analysis using DSC. Is there any explanation for these broad and flat exothermic peaks?

I am performing battery thermal analysis using DSC. I have tested there samples for 3 heating rates, 10, 15 and 20 deg Celsius per minute.

07 January 2024 8,218 5 View

Can anyone explain how do we deal with stationarity on case of unbalanced panel data where N>T?

For example some of the variables in the model are stationary at level and others are not. how do we deal with that.

21 June 2023 1,105 4 View

Panel ARDL or GMM?

I am working on long panel with N=365 and T=8. My model is that DV is a function of Independent variable, lagged value of Independent variable and lagged value of Dependent variable. DV=...

27 December 2022 2,462 4 View

What is the authenticity of turnitin plagiarism report?

The plagiarism is great concern for researchers, in such a situation the plagiarism checking software are helping hand. Is report of such software trustworthy. What is the authenticity of...

06 June 2021 6,732 10 View

Any suggestions on how to use commercially obtained SERS substrates to enhance Raman signal?

We have a 1mw-100mW 785nm Raman spectrometer. We have tried many commercially available SERS substrates (paper and AuNP). We are unable to see any Raman signal. Ideas on what we may doing wrong or...

25 January 2021 3,002 5 View

What is the role of top counter electrode when using perovskite thin-films in photoelectrochemical application?

I am currently working on perovskite based photoelectrodes, but I do not want to replace the counter electrodes including Ag, Au, will it have serious issue in the PEC performance?

13 January 2021 3,602 2 View

Which statistics to be used?

To study the affects of gender on the level of anxiety among college students. If I have gender as an independent variable and anxiety variable (with 3 factors) as dependent variable- can I use...

18 December 2020 7,198 11 View

I am interested in locating nutrients essential for photosynthesis. How can I see the chloroplast of lemongrass leaf using SEM ?

I am interested in locating nutrients important for photosynthesis. How can I locate them in lemongrass leaf using SEM or some other protocol.

07 July 2020 6,973 3 View

Geotechnical Engineering (Proceedings of the ICE) time review?

Hello everyone, I recently submitted an article to Geotechnical Engineering (Proceedings of the ICE), and the current status has been listed as "EiC Pre-assessment: Ready" for the past 20 days. I...

10 August 2024 6,493 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Research Methodology - Impact of Corporate Reputation on Stakeholders Behaviors?

Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks

06 August 2024 949 0 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

Should I remove an item from a scale to raise Cronbach's alpha and McDonald's omega or is it better to leave it if they are both over .7 already?

Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...

01 August 2024 4,606 7 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

Cash Kumar

ok , thats nice to listen and i am happy to help. but are you also concern about HYDROXY methylation nad is it about MSP/BSP ?

Gautam Pandey

Try MethPrimer primer designing tool..All the best !!

Aanchal Mehrotra

I am trying to use Methylight PCR technology using 2 specific primers and a taqman probe, i think this Methprimer design tool just gives me primers for MSP and not taqman probe sequence.