Gautam Pandey

4 Questions 13 Answers 0 Followers

Questions related from Gautam Pandey

Knowledge required for the generation of stable cell line of HepG2 cells?

I am making stable cell line of HepG2 cells after transducing them with shRNA-Lenti virus. I have done a kill curve and used 2 ug/mL of Puromycin for the selection pressure. I can identify now...

30 March 2021 348 0 View

Is Heat inactivation of Serum necessary for the growth of 3T3-L1 adipocytes?

I am using 3T3 L1 adipocytes and I found that most of the times they detached during later stages of differentiation. While figuring out everything, I am little susceptible to use heat inactivated...

09 June 2016 3,705 7 View

I am trying to induce lipolysis in 3T3 adipocytes by using Isoproterenol hydrochloride (I6504 Sigma). Why I am not getting any effect?

I have bought Sigma Isoproterenol Hydrochloride (I6504). I have dissolved it in KRBH buffer (pH-7.4) to make it 50 mM stock and stored @ -20. I used 10 micromolar conc. to treat adipocytes for 1-2...

30 November 2015 3,334 12 View

Phosphorylation of tyr or ser residues in a protein can activate or deactivate it despite both amino acids being polar. Can anybody explain this?

For example, take the case of Insulin Receptor and Insulin Receptor Substrate.

14 May 2015 7,579 2 View