Gautam Pandey

2 Questions 11 Answers 0 Followers

Questions related from Gautam Pandey

Knowledge required for the generation of stable cell line of HepG2 cells?

I am making stable cell line of HepG2 cells after transducing them with shRNA-Lenti virus. I have done a kill curve and used 2 ug/mL of Puromycin for the selection pressure. I can identify now...

30 March 2021 297 0 View

I am trying to induce lipolysis in 3T3 adipocytes by using Isoproterenol hydrochloride (I6504 Sigma). Why I am not getting any effect?

I have bought Sigma Isoproterenol Hydrochloride (I6504). I have dissolved it in KRBH buffer (pH-7.4) to make it 50 mM stock and stored @ -20. I used 10 micromolar conc. to treat adipocytes for 1-2...

30 November 2015 3,255 12 View