No title

Hello, I am looking for the original version of COHIP. To measure quality of life related to oral health, in children.

15 June 2020 5,166 1 View

How to do regression analysis in SPSS when the independent variables are manipulated (I only measuring the dependent variables in the survey)?

So in my research the independent variables are manipulated online review content (manipulated valence, platform, etc). There are 6 different groups with different manipulations (conditions). And...

14 July 2019 3,996 3 View

What is the best instrument to check on elements of lubricating additives? Using types of chromatography or any engine test?

To know the elements in PIBSI one of the additives for lubricants

08 May 2019 1,111 3 View

How to do miRNA isolation from hematopoietic stem cell with good purity?

Hi, currently I am isolating miRNA from hematopoietic stem cell using miRNeasy Qiagen kit. However, I didnt get good purity for both A260/280 and A260/230 plus low concentration as well. According...

14 October 2018 6,123 1 View

Can someone help me with sample size?

Here is the case: comparing systolic blood pressure (in mmHg) in a trial of new drug and placebo. Now I know that the formula of minimum sample size for this case is: 2.((Za + Zb).SD/d)2 Red: 2...

18 January 2017 8,525 5 View

How to determine the optimum voltage and time for a better separation in gel electrophoresis?

28 July 2016 4,436 3 View

Is no problem if I do Ethanol precipitation with composition glicogen, Na-asetat, before and after phosphorylation PCR product by T4 PNK ?

Dear all, Is there anyone have experience do ethanol precipitation PCR product prior to phosphorylation by T4 polynucleotide kinase? According literature ethanol precipitation by ammonium asetat...

26 July 2016 1,035 3 View

How do I grow single cell become colonies after sorting by FACS in mcf7 cells?

I have already prepared knock out cell by using px459 for knock out mcf7 cell then shorting by using FACS to get single clone, one day after FACS I get around 10 single clone/well ( positive EGFP...

20 December 2015 7,935 6 View

Is there a possibility that the virus accepted by the cells after several days was rejected from the cells, in a stable transfection of a cell line?

I had injected retrovirus containing pBabe.puro.cmv and pcl ampho to the F1 B16 and then after 24 hours and 48 hours I did puromycin selection, The First days and second days the cell decrease...

01 April 2015 3,936 12 View

How to modeling shear failure in abaqus?

Hello. I am using Abaqus to get the maximum shear force that can be carried by concrete beam without transverse reinforcement. I use concrete damaged plasticity and static-riks. But when the...

01 January 1970 2,226 5 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

25 February 2021 5,712 3 View