I am using murine cell lines for my study. I need to design primer of some miRNA which are not available commercially. Can anyone suggest website and published papers for how to do it.
I already have read the post. I tried the mirprimer2 software. It gave primer seq, but the prob is all reverse looks like same so I got confused if it will work or not. and agilent primer guideline does not have any solve about reverse primer. actually need someone actually used one of them. If you use their guideline and it work then tell me.