How to design/choose an effective primer for RT-qPCR?

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Can I dilute, lyophilised LPS and then store them as aliquots?

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Which house keeping genes I can use for standard and to normalize the expression of genes of interest (in Real Time-qPCR, gene expression analysis)?

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Sample dilution for quantitative analysis using ELISA?

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Rajendra K Gangalum

DNA man, prime3 and many more are available online. I suggest you to make primers in exon -exon boundaries if are performing reverse transcription-qPCR. You should be

Careful with AT to GC ratio,not to exceed 50-55%. Avoid primer diner/self annealing.

Rkg

Oskan Tasinov

I'm using cDNA to perform Real Time - qPCR. I work on gene expression of antioxidant and inflammatory related enzymes.

Thank you for suggestion!