Oskan Tasinov

7 Questions 26 Answers 0 Followers

Questions related from Oskan Tasinov

Which values are used for calculation of p-value (e.g. in t-test) if we use 2^-ΔΔCt method for calculation of gene expression?

We have a group of volunteers attended an intervention study. Samples were taken before and after the intervention and RNA was isolated to analyse change in gene expression, using qPCR. So in...

01 January 2019 5,809 3 View

Could someone suggest a cell culture model for analysis of glucose uptake?

I am planning to analyze the glucose uptake in 3T3-L1 cells. The suggested treatment scheme will be used to analyse the effect of plant extract on glucose uptake. Control groups...

10 October 2013 5,628 8 View

J774A.1 macrophages - Which are the best concentration of LPS (E. coli 026:B6) and treatment time to get good induction of inflammation?

Should I perform MTT test to analyse the effect of increasing concentrations of Lipopolysaccharide (LPS), before induction of inflammation with one specific concentration? I can choose a treatment...

09 September 2013 502 9 View

Can I dilute, lyophilised LPS and then store them as aliquots?

1. In what conditions/temperature aliquots should be stored? How long they can be stored? 2. Can I use other diluents except H2O? 3. Could someone recommend me a protocol/s for 1. and 2.

04 April 2013 1,521 23 View

Cultivation of human PBMC? Protocol for treatment of PBMC's with LPS?

1. Could someone recommend a culture media for human PBMC cultivation? 2. What concentration of LPS should I use to induce inflammation in cultured PBMC? I found that concentrations between...

04 April 2013 3,229 21 View

How to design/choose an effective primer for RT-qPCR?

Can you suggest a good and easy software? What should be the best characteristics of the primer? What should I keep out? Many of primers found in literature and databases, did not work as...

02 February 2013 9,778 2 View

Which house keeping genes I can use for standard and to normalize the expression of genes of interest (in Real Time-qPCR, gene expression analysis)?

I work with 3T3-L1 preadipocytes and J744A.1 macrophages. In recent gene expression analysis as standard in RT-qPCR I use b-actin gene. Now I should analyse gene expression in PBMC isolated from...

11 November 2012 7,657 41 View