How to evaluate the ezyme function using molecular dynamics simulations or other theoretical methods?

I would like to assess few enzyme proteins among the organism to understand the difference in their actions. The structure was modelled using homology modeling and same enzyme having different...

04 May 2019 6,329 8 View

Submission of new ssDNA sequence to database-APTAMER.?

Dear Researchers, We synthesised a novel aptamer against a particular protein/peptide target. Its a ssDNA with 87 bp and we would like to submit it to any database such as NCBI. Can anyone tell me...

10 November 2018 295 1 View

Blood delivery or bbb penetration in vivo procedures?

Can anyone share simple procedure to evalute the brain delivery or blood brain barrier penetration of a molecule. We dont have much facilities so basic or simple procedures are welcome. We would...

08 September 2018 3,395 0 View

Mutations by molecular dynamics simulation?

07 August 2017 3,012 2 View

Docking and missing residues of proteins?

Is it necessary to add missing residues in proteins before performing docking and MD

08 September 2016 4,071 8 View

Molecular dynamics of Modeled protein?

What is the significance of doing MD of modelled proteins. ?

08 September 2016 1,989 3 View

How can i calculate water permeability using Gromacs?

06 July 2016 9,899 6 View

How to Select a PDB file from Protein Data Bank

08 September 2015 8,658 2 View

How to define energy minimization parameters for a cyclic peptide? GROMACS

how and what are the files need to be defined? any manual?

05 June 2015 2,947 7 View

Gromacs Charmm27 forcefield adding residue help?

In picture given atom and atom type ,what type of naming is it and how i can make this sort of naming from my structure ,what is this A and B etc... I'm from a physics background and I am not...

05 June 2015 4,609 4 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

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23 February 2021 4,014 3 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

No title

22 February 2021 9,932 11 View

Can we remove the fmoc group after peptide cleavage?

I have synthesized the peptide using Fmoc based solid phase peptide synthesis. Also I have cleaved the peptide from the resin and lyophilized it, but later realized that, I forgot to deprotect the...

21 February 2021 4,537 3 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

Is there a method for evaluating nanoparticle adhesion to epithelial tissue in vitro?

I want to measure how well nanoparticles can transfect cells based on NP adhesion properties. In the literature I find people do it in animal studies, and epithelial adhesion helps in transfection...

21 February 2021 7,791 3 View