Can anyone share simple procedure to evalute the brain delivery or blood brain barrier penetration of a molecule. We dont have much facilities so basic or simple procedures are welcome.
We would like to perform in vivo experiments in mice.
I would like to assess few enzyme proteins among the organism to understand the difference in their actions. The structure was modelled using homology modeling and same enzyme having different...
04 May 2019 6,498 8 View
Dear Researchers, We synthesised a novel aptamer against a particular protein/peptide target. Its a ssDNA with 87 bp and we would like to submit it to any database such as NCBI. Can anyone tell me...
10 November 2018 445 1 View
Hi, How i can design an aptamer for a particular protein/peptide in silico, which help to reduce SELEX time and costs.
03 April 2018 3,468 3 View
Dear All, I want to study the role of an amino acid in a protein by mutating it to another amino acid. How will decide or select, the new amino acid? What are the parameters I need to consider?
07 August 2017 3,179 2 View
Is it necessary to add missing residues in proteins before performing docking and MD
08 September 2016 4,295 8 View
What is the significance of doing MD of modelled proteins. ?
08 September 2016 2,143 3 View
Most of the Literature i have seen with NAMD.is there any method or tools in Gromacs to study water and ion permeability of channels in gromacs? from trajectory or otherwise
06 July 2016 10,065 6 View
Hi, For the Molecular dynamics work how and o what pdb of particular protein i should select Example When i search for lysozyme many are coming with the codes 1JUG,1JKB so what are these codes and...
08 September 2015 8,910 2 View
how and what are the files need to be defined? any manual?
05 June 2015 3,132 7 View
In picture given atom and atom type ,what type of naming is it and how i can make this sort of naming from my structure ,what is this A and B etc... I'm from a physics background and I am not...
05 June 2015 4,780 4 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...
06 August 2024 726 3 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...
04 August 2024 4,725 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View