Papori Buragohain

3 Questions 7 Answers 0 Followers

Questions related from Papori Buragohain

How to solve the appearance of higher sized dsDNA products forming during aptamer SELEX?

I am working with a N20 mer (20 mer random region) aptamer library with a total length of 56 bps. I am facing the problem of higher sized ds DNA PCR products forming in my SELEX cycles. The size...

05 May 2018 918 3 View

Why does the ITC curve in an interaction between a DNA aptamer and peptide ligand have both exothermic and endothermic peaks at the same time?

I am using MicroCal ITC 200 (GE) and performing an interaction study between a DNA aptamer (12 kDa) and a peptide ligand (1.91 kDa). How can I explain an ITC curve with both negative and positive...

12 December 2017 9,185 5 View

Quantifying Fmoc release in SPPS

Can anyone help me how to determine the level of attachment of amino acids onto Wang Resin (loading capacity 1.2 mmol/g) by quantifying the Fmoc release in SPPS?

02 February 2016 4,064 2 View