How to design a primer for the promoter region?

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Madhubala Sharma

Dear Laurence Stuart Dawkins thanks for your prompt reply.

I had a query that how to locate promoter region on a gene in NCBI, only then we can design a primer for it.

Shubham Goel

very simple ...first you locate transcription start site and upstream to 1st nucleotide of TSS is promoter region which is designated as minus (-). Now your next query would be that how to find out Transcription start site? if it is then i will let you know .

ok...... Thank you, sir :-) (Shubham Goel & Mukul Rastogi)