4T1 cells metastasize to different organs but don't as well to bone. 4T1.2 cell line metastasizes preferentially into bone. Is there a defined protocol for isolating 4T1.2 cells from mice inoculated with 4T1. I have gone through a few papers but it'll be good to get the information from someone involved in these experiments.
Please see this paper:
http://www.ncbi.nlm.nih.gov/pubmed/?term=lelekakis
Hi Bedrich,
Thank You for your response and for the link.
Best.
Lakshman
TQ Bedrich, the link you shared is very good to me also, long time I am looking for this answer. TQ again
You basically can't generate 4T1.2 cells from 4T1 cells de novo because 4T1.2 already exist. Even if you do the same protocol to isolate a subclone of 4T1 cells that have similar metastatic capacity as 4T1.2, cells you cannot call them 4T1.2 cells, you would have to call them something else. We have 4T1.2 cells and we procured it from Australia from the same group that made them. If you are looking to get 4T1.2 cells then I would suggest getting them from Robin Anderson's group from the paper posted by Bedrich.
Ken's statement is correct. You will not be able to isolate the exact same clone. If you do wish for 4T1.2 specifically you can request it from the original authors. Still, if all you are interested in having a more metastatic variant than 4T1, you should still be able to isolate it by single cell selection. According to Lelekakis et al, you should be able to isolate 2 clones in 13 that have bone metastatic capacity.
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