Lakshman Singh

4 Questions 8 Answers 0 Followers

Questions related from Lakshman Singh

Can anyone suggest a mouse cell line to be used as a negative control for Pax7 expression in C2C12 cells?

I am staining for Pax7 (fluorescence), and was looking for a negative control to establish the staining protocol. Any suggestion would be highly appreciated. Thanks a lot

11 December 2016 688 2 View

Can anyone suggest a protocol for C2C12 cell differentiation to myotubes?

We seem to get a lot of proliferation in C2C!2 cells, even when they are treated with 2% horse serum. Though we see differentiation, there are still a lot of newly dividing cells in our cultures.

05 October 2016 1,172 8 View

Can anyone suggest a holder for Leica diamond blades to be attached on a Zeiss Hyrax M40 microtome? I am wondering if that is even possible?

We want to use a Zeiss Hyrax M40 microtome for plastic sectioning. It has never been used for plastic, and we have Leica Diamond blades that we were thinking of using. Any suggestions whether this...

05 October 2016 5,358 2 View

How to derive 4T1.2 cell line from 4T cells?

4T1 cells metastasize to different organs but don't as well to bone. 4T1.2 cell line metastasizes preferentially into bone. Is there a defined protocol for isolating 4T1.2 cells from mice...

15 December 2013 9,903 6 View