I did an orthotopic breast cancer mouse model by injecting human breast cancer cells MCF7 into the mammary fat pad of nude mice (normal cells without any fluorescence marker). After I harvest primary tumors, I want to check if there is any metastasis appears in lymph nodes and spleens. So I want to know if anyone can share the criteria for detecting metastasis in those tissues if I can only use frozen section and H-E staining. Thank you for your help.
As you know, it is kind of difficult to detect metastasis of cancer cells in mouse model. So, researchers transfer some gene into cancer cells, such as GFP, RFP, YFP. to trace cancer cells transplanted to mice. In my case, human pancreatic cancer expressed CA19-9, which is clinically used as tumor marker. I think CA15-3, CEA, NCC-ST-439 are clinically used for human breast cancer marker. Also, E-cadherin is epithelial marker for cancer cells, not on lymph nodes.
Can you examine which marker expressed on MCF cells with PubMed?
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