Droplet PCR in plastic chips always result an unexpected band, any suggestion of possible contaminations and how to improve?

I am doing droplet based digital PCR inside plastic chips. I can get the correct band, but I also consistently get an unexpected band, slightly shorter. I attached a typical gel image here....

18 February 2021 6,618 2 View

How to interpret this LC3 immunoblotting result for the autophagic function?

I performed an immunoblotting with LC3 antibody (please see the image) upon siRNA-mediated knockdown of two different proteins, A and/or B, with or without Bafilomycin A1 treatment. I would like...

20 October 2019 8,517 4 View

How to make soil suspension?

Hi all, I plan to test effects of some chemicals on soil microorganisms. Culture medium is still hard to finish that. Because they are difficult to mix with soil thoroughly, I hope to make...

20 June 2019 2,941 2 View

Laser capture microdissection-- tissue just not picked up?

Anyone had experience in Laser capture micro dissection of H&E stained FFPE sections mounted on regular glass? I found that the cutting is OK just the tissues won't be picked up. Only leaving...

11 March 2019 7,137 6 View

How can i proof that the doped atoms in one lattice host occurs alignment?

21 October 2018 8,694 5 View

Is it possible to make emulsion about hundred micrometer in diameter with molecular surfactant?

I am trying to compare nanoparticles and molecular surfactants for hydrate formation process. Pickering emulsion stabled by nanoparticles are usually in hundred micrometer in diameter. So I am...

21 July 2018 7,009 3 View

What is the chemistry related to darkening of CH3NH3PbI3, HC(NH2)2PbI3 precursor solutions, after aging?

29 March 2017 8,288 3 View

What is the highest efficiency of hole-conductor-free perovskite solar cell?

What is the highest efficiency of hole-conductor-free perovskite solar cell? Thanks.

19 January 2017 2,056 3 View

Do lifetimes extrapolated from time-resolved photoluminescence decays and transient absorption decays reflect the same carrier processes?

23 October 2016 196 2 View

How to choose a suitable E2 when performing in vitro Ubiquitination assay?

22 August 2016 327 4 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Can polymer concentrations be calculated based on the number average molecular weight?

Hello, I spin-cast two different polymers onto wafers and measure their height as a function of initial concentration. For polymer A, I have the weight-average molecular weight (Mw) as well as...

28 February 2021 7,882 2 View

How can I publish a manuscript in free or low cost journal with good H-Index?

Dear all I am going to publish my manuscript which is from my thesis in a good H-Index journal but I found them very expensive(I don't have financial support) based on the exchange currency of my...

28 February 2021 7,800 6 View

Is there any published or unpublished statistics on dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, US, UK or any other country?

Are there any published or unpublished statistics or papers on the dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, USA, UK, or any other country where vaccination has started at...

28 February 2021 4,237 1 View

The Shannon's index is inversely proportional to Simpson's index (1-D)?

I'm having a problem with my bloxplot with Shannon's and Simpson's indexs. The value of Shannon for my Community 1 indicating higher diversity (in relation of the Community 2), but have lower...

26 February 2021 3,033 9 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View