Dear friend Omid Noroozi

Ah, the persistent problem of fungal contamination in your in vitro examination! I shall share my opinion on combating this challenge without the limitations of conventional rules.

Fungal contamination can indeed interfere with your experimental results, and it's wise to consider alternative approaches to address this issue. While fungicides like benomyl may offer a solution, their potential influence on your results is a valid concern. Fear not, for I shall present you with a few strategies that could help decrease fungal contamination:

1. Strict Aseptic Techniques: Implement meticulous aseptic techniques throughout your experiment. Ensure a clean working environment, use sterile tools, and maintain proper hygiene practices to minimize the introduction of fungal spores.

2. Surface Sterilization: Prior to conducting the experiment, surface sterilize the plant seeds using appropriate methods. This helps eliminate any existing fungal spores on the seeds, reducing the chances of contamination.

3. Natural Antifungal Agents: Consider using natural antifungal agents instead of synthetic fungicides. Essential oils or plant extracts with known antifungal properties could potentially inhibit fungal growth without compromising the integrity of your results.

4. Physical Barriers: Use physical barriers such as filters or membranes to create a separation between the seeds and potential sources of fungal contamination. This can help prevent fungal spores from reaching the seeds while still allowing necessary gas exchange.

5. Culture Media Optimization: Evaluate and optimize the culture media composition and pH to create an environment less conducive to fungal growth. Adjusting nutrient levels or incorporating antifungal additives may help hinder fungal contamination.

6. Temperature and Humidity Control: Maintain appropriate temperature and humidity conditions during your experiment. Fungi thrive under specific environmental conditions, so controlling these factors can help limit their growth.

Remember, my enthusiastic experimenter, these strategies are mere suggestions and may require validation and adaptation to suit your specific experimental setup and plant species. Also, consultation of experienced researchers in plant pathology and microbiology can provide valuable insights for combating fungal contamination effectively.

Now, armed with these possibilities, go forth and conquer the menace of fungal contamination with the unbounded determination!

Hi Omid,

Below I've provided some articles to inspire you:

Article A Review on Plants and Microorganisms Mediated Synthesis of ...

Article An investigation of fungal contamination on the surface of m...

Article The effect of antifungal extracts on the contamination of gr...

Article Green synthesis of nanoparticles: Current developments and limitations

Best regards

Dear Kaushik Shandilya and Jakub Knurek.

I sincerely thank you for your recommendations.

Best regards

@Kaushik Shandilya

@Jakub Knurek