How to decrease pH value of emulsion

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Minimum concentration of protein to be injected for detectable peaks of protein in 16/60 Sephacryl S-100 HR column for SEC-FPLC?

Hello Peers, Warm wishes to all. I am a new user of the Cytiva AKTA Start system for SEC with a column, HiPrep 16/60 Sephacryl S-100 HR (120 ml column volume). I am not sure about the minimum...

03 June 2024 7,713 2 View

How to split molecules into test and training set in 3D-QSAR?

Hi peers and experts! I am new to 3D-QSAR and tried to build a model based on 34 molecules, split into 10 test and 24 training sets. In the end, a poor model is evident from r2 and q2 values....

08 April 2024 2,785 6 View

Vitrogel or matrigel ?

As per practicle experience in oncology studies Vitrogel (https://www.thewellbio.com/product/vitrogel-3d/) or Matrigel...

24 January 2024 3,395 2 View

Cisplatin MC38 C57 mice ?

Hi Everyone, What should be the cisplatin doses for DRC in MC38 cell line inoculation in C57/BL6 mice? Best, -Abhijeet

04 January 2024 8,737 0 View

Do MCF-7 cancer cells forms tumor after SC inoculation in nude mice?

If "YES", how long it take to grow and if "NO" then why?

14 December 2023 698 4 View

Why same cancer cell line shows different growth kinetic in different strain of immunocompromised mice?

Is there any specific reason not to change strain of the mice for the same cell line?

12 December 2023 5,586 5 View

What is Repeated Earthquakes?

What is the concept of repeated earthquakes? How does the dynamic analysis is performed under repeated earthquake? Is this done in a different way that IDA analysis? How the ground motion data...

02 November 2023 4,884 4 View

While doing simulation on SCAPS-1D for the CdTe/CdS solar cell, I am getting a negative QE for higher wavelength range?

I required some data for the study related to QE of solar cell, but the problem is that I am getting negative QE values at higher wavelength range or at photon energy less than the band gap of...

11 October 2023 5,729 0 View

Does the results obtained with the cracked version of SAP2000 match with licensed version of SAP2000?

I am trying to verify the results obtained by modal analysis and response analysis of a G+4 RC hill Building with the base paper results but unfortunately couldn`t. Although I have followed all...

09 October 2023 7,390 9 View

Why my Ni structure is not converging after applying a strain in X-direction?

Please find the attached Ni Quantum Espresso Input file. &CONTROL calculation = 'scf' verbosity = 'high' restart_mode = 'from_scratch' tstress = .true....

11 September 2023 9,925 0 View

How to assess the osmotic power of a substance?

Hello, I suspect that a molecule I am using is causing bacteria to experience osmotic shock. What chemical properties should I look for to compare this capability with those of other substances?...

06 August 2024 977 1 View

How to check pH of a high strength hydrogel?

We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...

30 July 2024 942 2 View

EDTA buffer solution?

Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.

29 July 2024 4,374 1 View

Whether i can preserve leaves samples in 2.5% glutaraldehyde in 0.05 M phosphate buffer, pH 7 for coming several weeks for further LM study ?

I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.

29 July 2024 8,560 0 View

How can I regenerate a SCX column modified with Sulfonate group (Thermo 054995)? The colmun has lost its Resolution and peak to valley ratio?

The column is used in Ion Exchange Chromatography of Monoclonal antibodies and the method of analysis is based on pH gradint.

13 July 2024 7,462 1 View

How i figuer out the weight of curcumin powder?

I am trying to load curcumin emulsion in 4% chitosan gel but i don not know how many mg powder is needed ?

10 July 2024 2,462 1 View

My Cyclic voltammetry curve doesn't show cathodic peak of Cu2+ to Cu+. how to solve this problem?

I performed CV of Cu-MOF in a buffer solution of pH 8, and we did not find any peak corresponding to Cu2+ to Cu+. What is the reason behind this? Please give your valuable suggestions.

08 July 2024 2,388 4 View

Is it possible to generate a (semi-)permanent "transcription bubble" within a replicable plasmid?

I was wondering if it is possible to form a permanent open "ssDNA bubble" similar to a transcription bubble (>13 nucleotides) within E. coli. These criteria are important: 1. Open ssDNA...

07 July 2024 8,275 5 View

What is the best way to get rid of unpleasant odour of acrylic emulsion beside redox post polymerization?

Even adding fragrance is not working

05 July 2024 741 2 View

Transparency oin-in-water emulsion but larger size particle ?

Dear Colleagues, I am currently formulating oil in glycerin/water nanoemulsionn of Thymol and Eugenol, using cationic surfactant. After phase diagram, I got trouble that the clear and...

03 July 2024 4,200 2 View