In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

What is the suitable range of Sulfur content and Viscosity of Heavy fluid Oil that used in Kiln?

What is the disadvantages of high sulfur content to the ceramic burning process? and what is the effect of outranged viscosity?

24 February 2021 8,219 2 View

How to do ORR test with pine(Which means RDE or RRDE electrode)?

I want to do LSV ,CV test of ORR(Oxygen reduction reaction). But our laboratory don't have the RDE and RRDE electode. And I was wondering if I can do the tests by magnetic stiring or mechanical...

14 January 2021 1,869 2 View

Is it possible to add hierarchical porous Cu/Zn-nanoparticle@carbon materials into a carbon paper based gas diffusion electrode (GDE) ?

26 November 2020 5,075 1 View

How to reduce (which reducing agent) a disulfide bridge in a protein and how long does it remain reduced?

I want to reduce the disulfide bridge in my 7kDa protein. My protein has only 1 cysteine at the surface and thus makes a homodimer using a disulfide bridge. I want to reduce the disulfide bridge...

24 November 2020 3,211 5 View

How can i define a correct baseline for reading the anodic (forward) peak current when oxygen reduction reaction is distorting my cyclic voltammogram?

Dear colleagues I have troubles to read out the correct anodic peak currents from my cyclic voltammogram since ORR is pushing down my baseline. Is there anything (besides substracting a proper...

23 November 2020 4,654 6 View

Can I use a Glass electrode (standard used for pH measurement) for measuring ORP?

I have made a test to investigated: I measured the potential in mV of a standard +220mV solution using my pHmeter which gives a mV value with the pH. The result was +9mV. But this was the result...

06 October 2020 7,220 3 View

What is the major meaning of PCs in Principal Component Analysis?

I am using PCA to extract major meaningful data from many different parameters that are embedded in my remote sensing satellite imagery. I know that PCA is a dimension reduction model and the...

13 September 2020 7,596 7 View

How the nature of ring electrode of RRDE is known to influence the percentage of peroxide detected during ORR reaction?

Why mostly GC disc and Pt ring RRDE is preferred for the detection of peroxide during ORR reaction? What will be the effect if the other ring electrode is used instead of Pt.

10 September 2020 1,558 2 View

Need airfoil coordinates?

Hi all, I hope you all are doing well. I am working on the simulation of airfoils with boundary layer suction. I found a reference to validate my CFD results, as shown below. "Stanewsky E,...

06 September 2020 9,013 3 View

I am searching for "Model order reduction of nonlinear systems". how can it be possible?

I have a complicated 4th order nonlinear differential equation for a system. I am going to design a nonlinear controller for this system. is it possible to reduce it into a second-order system?...

28 June 2020 930 14 View

Can anyone share with me a detailed protocol for performing a Plaque Reduction Assay to test anti-viral activity?

I am working with a team looking into testing antiviral activity of naturally occurring substances. We have not decided on the specific protocols but I am leaning towards the plaque reduction...

18 April 2020 762 3 View