Nanopore MinION produces .fast5 files as raw data. How to convert .fast5 file into fastq format?
There is a software to convert fast5 files into fasta, fastq files called poretools. Have a look here for installation and example of usage:
https://poretools.readthedocs.io/en/latest/
There is a software to convert fast5 files into fasta, fastq files called poretools. Have a look here for installation and example of usage:
https://poretools.readthedocs.io/en/latest/
Dear Abdul,
Fast5 files have the signal obtained from the nanopore. To obtain fastq files you need to perform a basecalling. To do so, you have several software that have different efficiencies. Please take a look at the following comparison:
https://github.com/rrwick/Basecalling-comparison
Hope this helps.
All the best,
pore tools wil do nothing for you. its not a question of data conversion, its a question of Basecalling from raw signal into nucleotide sequence. Essentially a fastq file is just a text file that is human readable. If you are not interested in basecall quality, you may convert to fasta to just provide the read name, and its basecall sequence.
For basecalling, your best bet is guppy, downloadable from main community page following the documentation.
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