How many mice are needed for long term potentiation?

As above, we are trying to do the Long-term potentiation test using mice hippocampal slices. How many mice are needed or how many slices are needed for LTP? Can anyone help me with that?

15 February 2020 7,073 3 View

Does anyone happen to know how to deal with kernel random matrix without using any expansion (i.e., Taylor, series, orthogonal or bi-orthogonal)?

The literature all depends on some kind of expansion. It is useful for global analysis but not for local.

28 January 2019 6,589 1 View

ARIMA, R square and stationary R square?

Dear all When ARIMA model was used to make the unstable time series data stable. There are many model to choose. which is the best one? Not only BIC value was shown, but also R square,...

24 December 2018 7,782 3 View

Aberrant protein after crispr/cas9?

Hi All, I used Crispr/cas9 to knockout CISH in T cells. The T7EI assay showed the gene editing. However, when I used the Western blot to check the protein expression, I found the the bands with...

12 November 2018 3,755 4 View

Is there any textbook or technical instructions on the preparation of ceramic powder and sample?

Dear all, I am to do some experiment on ceramic powders, alumina for example and wondering how to prepare the powder and sample. I wish to have some recommendations on textbooks and technical...

04 March 2018 4,077 4 View

How to stain RNA for EM?

Does any one know a good reagent (or protocol) to stain nucleic acids (RNA) for electron microscopy (either negative stain or positive stain)? p.s. This is for single particle analysis. Thanks!

31 October 2017 3,663 3 View

HOW TO DO ATRP REACTIONS ON THE SURFACE OF SiO2 NANOPARTICLES?

I encounter two questions during SI-ATRP. My experiment route is shown as follows. First ATRP reaction, MSN-Br(100mg), GMA( Glycidyl methacrylate, 1.5mmol),...

23 December 2016 3,066 3 View

Are deep neural networks practical to problems with low-dimensional input data(e.g. under 10 dimensions) ?

Deep neural network has been proved that efficient in imaging, text and voice data. Are they work good in processing low dimensional data ? (One example, using the DNN to analysis mechanical...

19 May 2016 3,367 3 View

How to do protein purification by intein tag/chitin beads in mammalian cells?

17 April 2016 1,304 2 View

What's the difference between the polarization direction, maximum radiation direction and direction of the electric field distribution?

10 February 2016 7,955 2 View

Discrete Phase model (DPM) particle break-up has particle size limits during particle tracking?

Hello, I am currently working on simulating water droplet partciles in a closed room. When I use uniform particles, without "particle break-up", I can carry out the simulation without any problem....

02 March 2021 3,053 2 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

Is there a deep reason for using the squared modulus of the wave function as the probability density of the existence of the particle?

I think we were all surprised at the first time we got to know quantum mechanics that the squared modulus of the wave function is the probability density of the existence of the particle? The role...

28 February 2021 6,282 9 View

Zno nano particles colour changed from white to grey in process of calcination any suggestions?

In the formation of Zno nano particles process, after drying at 120 degree C, my zno nano particles are completely white but when i calcine it for 450 degree C it converts into grey colour. i...

26 February 2021 2,435 4 View

Why there are two peaks while using the mie plot of Silver nanoparticle?

Hi, I am trying to learn the theoretical modelling of silver nanoparticle using the Mie scattering theory. After that, I discovered a software named Mieplot developed by Philip Laven, where it...

25 February 2021 7,493 6 View

Questio regarding micro/nano particles fabrication ?

I will start to fabricate micro/nanoparticles for growth factor release and I check out a procedure quite nice, however, it is lacking some details and since I don't have much knowledge doing this...

20 February 2021 199 2 View

Why is my pressure equal to kinetic energy in NVT MD simulation?

I don't know much about molecular dynamics, but I am suspicious of my pressure being equal to kinetic energy. I am using // sum the velocities squared: double v2sum = 0.0; for (int i = 0; i

15 February 2021 1,316 3 View

How complex systems physics/statistical mechanics would be helpful for particle physics & Networking?

Recently, I've been selected for an ICTP program named Physics of Complex Systems. But, I have a keen interest in Particle Physics & Quantum networks. As statistical mechanics involved in...

11 February 2021 699 3 View

What is the structure of a photon that can describe the interference experiments that reject the wave nature of light?

De Broglie's double-solution postulated a causal model of Quantum Mechanics. Knowledge of the photon structure was needed to model the particle part of the cause of the Double Slit interference...

11 February 2021 6,869 5 View

How can I take subsamples of water from a larger sample in a way that allows me to measure the iron content of the subsample?

I learned how to take subsamples with a magnetic stir bar, but I noticed iron particles stick to the bar, and I am having trouble finding a standard method to solve that problem. How can I take a...

04 February 2021 4,778 1 View