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Questions related from Naveen Sathyan
RG provides you with a platform to discuss the problems you face in you research work with experts around the globe. It surely has helped me tackle many obstacles related to gene cloning. Has it...
03 March 2014 6,963 10 View
These creatures were found attached to leaves of mango tree. This is the first time I am seeing these. Can anyone identify them?
02 February 2014 2,594 20 View
While attempting to write a paper everyone of us has a rough ride at one step or the other. Which one do you find the toughest....Deciding on the title, Introduction, analyzing the results,...
02 February 2014 6,481 10 View
Performing experiments on species with low population sizes raises serious concern as many experiments or studies require sacrificing the organism. Scope of publication in scientific journals...
02 February 2014 513 6 View
In modern era, competition is becoming more and more intense. Does it encourage a scientist to put in greater effort toward solving problems or does it have a negative impact? In your opinion what...
01 January 2014 262 15 View
Generally 90% of a researcher's efforts end in negative results. The remaining 10% of his/her results are positive and it is shared with the world in the form of publications or patents. Negative...
01 January 2014 4,924 46 View
The main aim of research should be either to gain knowledge or to solve a problem and to work for healthy development of mankind and nature. Nowadays researches are planning their work, so as to...
12 December 2013 421 97 View
Why is it that many bacterial strains become more virulent, have reduced lag phase and increased exponential growth rate when exposed to reduced gravity as in space stations? Does it have an...
11 November 2013 6,690 1 View
Can a phage transform a non virulent bacteria into a virulent form. Are there any examples?
11 November 2013 5,847 8 View
I am using 10 microlitre of test sample, 10 microlitre of microbial suspension (in hepes, adjusted to 1000 bacterial cells), and 80 microlitre of MH broth in a 96 well microtitre plate. Reading of...
11 November 2013 5,388 4 View
We are all aware of the evils of pollution and its impact on ecology. Among all the destruction it causes, is there any chance that it may actually lead to evolution of some species? Are there any...
10 October 2013 6,508 34 View
I have got transformed Rosette gami cells. I have used pET vector containing histone protein insert. If I seed these transformed cells on IPTG incorporated LB agar plates, will the cells start...
10 October 2013 1,799 10 View
Corals and sponges are considered to be gold mine for secondary metabolites. In our hunt for novel bio-active natural compounds are we not destroying corals and sponges? For purifying one gram of...
10 October 2013 2,505 4 View
I carry out ligation reaction at 22 deg C for one to two hours using T4 DNA ligase and then directly use the reaction mix for transforming DH5 alpha cells. I would like to know if inactivating T4...
10 October 2013 7,308 17 View
There are several reports on bacterial fossils and organic matter from meteorites. Bacterial spores can remain viable in very harsh conditions. Taking these reports into consideration, could it be...
10 October 2013 7,326 47 View
I want to send a few of my peptide samples for MALDI-TOF analysis. I would like to know how much of protein is required for MALDI analysis? Will it be safe to send the cut pieces of SDS-PAGE...
10 October 2013 1,503 6 View
Over-exploitation of fishery resources mainly in the marine sector is a social, economical and environmental curse. What steps need to be taken to ensure sustainability of fishery resources and to...
10 October 2013 9,443 11 View
I have purified my recombinant protein (antimicrobial peptide) using Ni-NTA column. I used 8 M urea having pH 4.5 for elution. If dialysis is not my choice, what would be my best option to safely...
10 October 2013 2,212 2 View
I got a few colonies of this particular organism as contamination on my LB agar plates stored at 4 degrees C. Please see the attached image of colonies. I have never seen this type of colonies...
10 October 2013 4,670 9 View
I have learnt that some natural foods like peas, tomatoes, mushrooms etc contain high concentration of glutamate. If that is the case then is mono sodium glutamate that much of a problem when...
10 October 2013 6,829 9 View
How can I preserve blood cells (from elasmobranchs) for RNA isolation if -80 Celsius degrees deep freezers are not available?
10 October 2013 1,953 6 View
Which is the best method for long term preservation of transformed Rosetta-gami (DE3)pLysS? Will it expel the pET vector with insert, if I keep them at 4°C for a week?
09 September 2013 1,847 4 View
I would like to do MALDI-TOF analysis of a few peptide samples. I have done tricine SDS-PAGE of the samples and would like to preserve them for MALDI-TOF analysis. How can I preserve the gel...
09 September 2013 1,167 1 View
In your opinion, which is the biggest hurdle that might impede the development of AMPs as commercial therapeutic agents? How to overcome it?
09 September 2013 4,420 3 View
I am a beginner in protein-protein interaction studies. I have started using ClusPro 2.0 and would like to know how efficient it is in comparison to Autodoc and other such softwares?
08 August 2013 399 2 View
