How to model the vapor plume during laser welding process using Fluent?

Problem description: I am using ANSYS Fluent (multiphase VOF & solidification and melting model) to simulate laser welding process. Is there any possibility of establishing a fully coupled...

05 November 2020 893 1 View

Should I add penicillin/streptomycin into cell culture media for doxycycline inducible system?

Hello, since I have no experience in Tet-on system in drosophila S2 cell culture, I need some technical help... Should I add P/S into the cell culture media? What's the doxycycline concentration...

17 September 2020 7,497 5 View

Why there is a 11 kDa band showing in SDS-PAGE of my IgG1 digestion with Papain?

Hi guys, I am using the papain digestion system to abtain a Fab. Here is my recent result, I got thin bands around 55, 45; thick bands around 30, 25, which may indicate incomplete digestion, but...

27 June 2020 3,882 9 View

Black paint for supercapacitor electrodes?

Do anyone knows what kind of materials could be used to paint a supercapacitor electrode as shown in the picture (the coated black shining materials)? Any help is appreciated. Thanks!

05 March 2020 2,194 4 View

Which short versions of Ryff's Psychological Well-Being Scale do you recommend?

I want to assess the well-being of farmers in Southeast through both an assets and psychological perspective and am having trouble finding a good measurement scale for the latter. Ideally, I want...

03 January 2020 1,927 3 View

Chinese research about post-industrial urban wilderness?

The meaning of abandoned spaces, especially industrial spaces, with over grown spontaneous vegetation have been widely studied in the Western world. I want to know is there any relevant Chinese...

29 April 2019 7,307 1 View

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I have a question about using stated-preference methods like conjoint analysis for valuing a landscape type - derelict industrial lands with spontaneous wild vegetation. As I have not found...

15 April 2019 1,498 4 View

What type of brain cell or tissue is this?

We recently discovered a probe could stain brain tissues and showed a very specific distribution, wondering what type of cells or tissue is this?

03 March 2019 7,214 6 View

Hey, does anybody know if there are some bacteria can use acetate to produce methanol or methylamine?

any bacteria can use acetate to produce methanol or methylamine? or use H2 and CO2 to produce methanol?

15 May 2018 9,401 5 View

Any one who is working on or interested in vision problems on videos?

I'm very happy to learn, discuss and collaborate on some vision topics on videos. If you are also very interested, feel free to contact me.

10 May 2018 4,869 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

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28 February 2021 9,936 2 View