I have amplified promoter (2000 bp)and its CDS(4000 bp) individually from DNA and cDNA respectively. I want to develop implementation vector.
I want to join my promoter and CDS before entering into vector.
Do i can use some ligase these two fragments? if yes please tell me
or any other easy method
Note: i cannot use Restriction enzyme as due unavailability of restriction site in my vector. So i want first get complete gene of interest.