04 April 2018 0 10K Report

I have amplified promoter (2000 bp)and its CDS(4000 bp) individually from DNA and cDNA respectively. I want to develop implementation vector.

I want to join my promoter and CDS before entering into vector.

Do i can use some ligase these two fragments? if yes please tell me

or any other easy method

Note: i cannot use Restriction enzyme as due unavailability of restriction site in my vector. So i want first get complete gene of interest.

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