I want to know the complete protocol of conjugation of COOH-PEG-NH2 with IONPs as I am not able to get the exact feasible protocol.
as iron oxide is negatively charged, and the amine group will be positively charged in aqueous medium , so just sonicate iron oxide with your compound in water. This what we did when we coated iron oxide with PEI which consist of amine groups.
Thanks and Please share the literature which you followed
Hi Amarjeet, i think this link can help you.
https://books.google.co.in/books?id=GDKgDQAAQBAJ&pg=PA210&lpg=PA210&dq=how+to+conjugate+the+iron+oxide+nanoparticles+with+COOH-PEG-NH2&source=bl&ots=8o7o441Ho1&sig=ACfU3U2-n0DlcFizJ-UeRwyBIv6_WZMHPQ&hl=en&sa=X&ved=2ahUKEwih8sT0rJLgAhUaUN4KHZgRDQ0Q6AEwA3oECAUQAQ#v=onepage&q=how%20to%20conjugate%20the%20iron%20oxide%20nanoparticles%20with%20COOH-PEG-NH2&f=false
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