I have generated a figure "Gene distribution by conservation" from 20 genomes of bacteria. Can someone explain how to interpret correctly?

I have generated a figure "Gene distribution by conservation" from 20 genomes of bacteria. Can anybody explain how to interpret it correctly? Thank you in advance

11 January 2021 3,346 2 View

How to calculate thermal stress in cylinder if water is flowing inside the cylinder?

How to calculate thermal stress in stainless steel cylinder if water is flowing inside the cylinder?

14 October 2020 905 2 View

How extract and purify extracellular proteins expressed in E. coli?

Hello everyone, I am having trouble isolating and purifying an extracellular protein which I have expressed in E.coli. I have induced my protein at 0.5 mM IPTG by incubating it at 37 degrees for...

02 October 2020 8,223 3 View

Systematic Review and Meta-analysis?

Hi, I am planning to conduct a systematic review and meta-analysis since very long. However, found difficulty in literature search and statistical analysis. Please anyone advice me any easy way...

11 July 2020 7,868 13 View

Can I use the same research instrument for collecting data from two different populations?

Can I use the same questionnaire to collect data from health as well educational sector employees to measure their level of job satisfaction ?

02 July 2020 7,480 1 View

Can anyone help me finding good impact factor journals to submit uninvited review paper on postharvest chilling injury in fruits.?

I have tried submitting my review paper in relevant journals, but most of them only accept the review paper invited by the editor of the journal

28 June 2020 4,371 5 View

How to calculate the sample size for a clinical study where the exact disease prevalence is not available?

While planning clinical study like RCT on diseases like COVID-19, how can we calculate the sample size when exact disease prevalence is not available

01 May 2020 5,079 3 View

What are the major problems and challenges in social science research?

What are the major problems and challenges in social science research and let us proposed comprehensive solution accordingly. The considerable known challenges are development of...

27 September 2019 1,757 13 View

Can SS 316L be heat treated at recrystallization temp. for achieving fine grains?

Will carbide phases get dissolved at this temp.? What can be the probable cooling rate? Please suggest

10 September 2019 5,188 4 View

How Research gate enhance our academic excellence?

Although, Research gate is a social networking site for scientists, social scientists and researchers to share papers, ask and answer questions, and find collaborators. But how it exactly enhances...

05 September 2019 8,889 30 View

Analysis of CRD two factor using R studio ?

Hello everyone. I have conducted the research on post harvest longevity of tomato crop using plastic packaging and different doses of chemicals as two different factors. The design of experiment I...

28 February 2021 8,189 3 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

How to measure 50uL of cells?

Hello, I am planning to do a transformation, and on the protocol it says "Scrape a 50 ml volume of yeast from the YPAD plate using a sterile inoculating loop or sterile toothpick". But I can...

14 February 2021 6,247 3 View

Cells not growing in large scale protein expression?

I am using KRX cell. I have grown my overnight cultures at 37 degrees. When I inoculate 1L TB media, my cells do not grow. I have also tried using 25 degree incubation of overnight cultures and...

10 February 2021 3,871 3 View

How to extract NLR genes from a database?

Greetings to all, I am not a bioinformatics person, but looking for a method that help me extract all NLR genes from benthamiana, tomato and soybean databases.. any suggestions so I can use my...

08 February 2021 4,557 2 View

Can I use the Chlorophyll stability index (CSI) to calculate the stress potential of cyanobacteria that are under stress?

I want to calculate the stress potential of cyanobacterial cells that are inoculated with antagonistic bacteria. My results showed that there is a drastic reduction in the chlorophyll content of...

23 January 2021 331 3 View

How to revive bacteria from glycerol stock?

19 January 2021 7,025 4 View

What should my objective ?

I am going to start my master program (msc agri hons) research in rull of potassium in tomato vigetable crop so what should my objective please guide me Thanks

13 January 2021 4,635 6 View

How ash helps in controlling pest,disease control ,is it good to apply or not ?

we all know that it is our traditional practice which we are performing in any problem in our kitchen garden to farm level is it good to apply or not, how it is good or what are its effects.

24 December 2020 2,699 15 View