I'm working with some hydrogel carrier systems, which carry 166Ho-based nanoparticles, aiming at brachytherapy applications. I want to perform some in vitro tests with radioactive systems to optimize my compositions.
However, I'm struggling with the methodology. I do not know whether the methodology I'm planning to use is the most appropriate for this purpose. In brief, I'm planning to seed cells in culture wells of a 24-well plate; then, I'm going to insert a transwell containing my hydrogels containing 166Ho in the culture plate. Later, the cell will be incubated for 48 h. Finally, I'll perform an MTT assay to evaluate the cell viability. (Please, see the attached paper in the "In vitro brachytherapy" section within section 4. Experimental Section).
If anyone has a better methodology or can suggest a specific standard to follow, I would like to hear some advice.
I really appreciate any help you can provide.
Hello Roger. I have never performed brachytherapy studies, but I am wondering if you can seed the cells in the cell insert and place the hydrogels at the bottom of the plate instead? That way your cells will grow in a more physiological relevant environment as they can access the media from both sides. Here you can find more info on how to select cell insert pore size, etc. Hope this helps! https://cellqart.com/downloads
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