How to compare results from quantitative MSP with results from TCGA cohort on RCC ?

More Antonios Koudonas's questions See All

How can I mix, compare, and optimize two or more DOE in Design Expert software?

I investigate 2 different geometries based on the same number of factors and responses. Factors' numeric values and ranges are the same for both cases, however, the responses' numeric values...

04 July 2023 5,819 1 View

Which is the proper way to do subgroup analysis between two different case-groups when the studies use the same control group for both of them?

In our meta-analysis, most included studies have two case groups (treated and untreated) and compare them with a single control group of healthy persons. In our paper, we want to study if early...

21 March 2022 1,909 1 View

Applying the Mundlak approach in 3-level hierarchical model. Which is the correct mean of a level-2 variable?

Hello! We have a question about implementing the ‘Mundlak’ approach in a multilevel (3-levels) nested hierarchical model. We have employees (level 1), nested within year-cohorts (level 2), nested...

14 July 2021 9,542 1 View

Design a taqman primers and probes for gene expression assay?

dear all as it is my first time to design a taqman assay , could you recommend for me a good reference to know the good designing for taqman gene expression assay, what is the accepted range for...

15 May 2019 8,367 2 View

Can Chloroform be used as a solvent in cytotoxicity test in cell culture instead of DMSO ?

my drugs can not be dissolved in any other solvent except the Chloroform, so can I use it on cells in MTT cytotoxicity test

09 December 2018 4,984 4 View

How can I estimate the shape and scale parameter of the Generalized Pareto Distribution (GPD) given a specific data-set?

I have already estimated the threshold that I will consider, created the pdf of the extreme values that I will take into account and currently trying to fit to this a Generalized Pareto...

18 May 2017 5,119 3 View

Except for longshore drifts, may we consider additional longshore currents which could potentially influence sediment transport and beach evolution?

Even if this not the case for natural beaches, I am thinking that mega-nourishments and artificial islands in particular might be exposed to additional currents to the longshore drift generated by...

19 March 2017 5,028 7 View

Best way to extract RNA from FFPE human brain tissue?

I am trying to compare yield of fresh vs FFPE and the measure relative quantity by RT-qPCR of housekeeping gene. I can upload the protocol I am using right now if anyone is interested in sharing...

11 December 2016 902 2 View

Do you know any estuaries which act as sinks of sediment material and cause beach erosion as well as problems to the coastal structures nearby?

I am looking for real cases of esturies where sediment material from beaches in their vicinity is trapped. As a result, coastal structures like groynes or ports near such estuaries may possibly...

25 September 2016 8,785 4 View

Best RT enzyme and best PCR enzyme for high GC content (>65%) RT-PCR?

I have thus used superscript II and IV :/ I denature RNA at 65 degrees, I wonder if I can increase that to a temperature at which DNA is usually denatured at (around 94 degrees) without...

31 August 2016 6,207 5 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Radiogenomics Cancer Research Challenges?

what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...

03 August 2024 5,828 4 View

Z-average size from Dynamic Light scattering can it be lower than the original probe size?

Hi everyone, Recently I have been conducting Dynamic Light scattering experiments in a micellar solution at 5 and gel at 37 degrees of Celsius with latex particles of diameter 190-500 nm. While...

01 August 2024 1,168 4 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

Dimensions of an MJ Research 96-well alpha module?

Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...

30 July 2024 2,867 0 View

How to build my own lab made four point probe set up?

Hello, I'm trying to measure the conductivity of semiconductor films but since I don't have a commercial four point probe set up I would like to build one on my own in my lab. I have generators,...

30 July 2024 906 2 View

How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...

30 July 2024 841 2 View