How to compare expression levels of different genes of the same tissue?

More Jie Yin Yee's questions See All

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

Alpha and dense granules in platelets not visible in Transmission Electron Microscopy?

I am trying to visualize alpha and dense granules in platelets. However, it seems like the granules were not stained well. Is there any way I can improve my staining technique to obtain better...

04 February 2021 5,925 9 View

How to measure temperature and humidity in a gel environment?

One of my project requirements is to measure temperature and humidity in the growing environment of plant. The plant will be grown inside a petri dish with nutrient gel. A waterproof temperature...

28 January 2021 9,415 2 View

How to combine multiple LEDs with different wavelengths to create a uniform illumination?

Hello, I'm a university student who is currently doing a plant incubator project, which requires LED grow light system. One of the project objectives is to provide a wavelength-tunable light...

25 November 2020 5,508 6 View

How do I apply a constant load with same velocity to reach desire deformation in ANSYS Workbench?

I'm currently working on my academic project and it is required to perform a simulation about ASTM D575-91 (Method B) using ANSYS. I'm totally new to ANSYS, hoping someone can help me out. "The...

28 October 2020 8,764 3 View

No title

How can I know the expression level of common tumor targeting receptors on cancer cell lines? For example the cell lines and receptors I mentioned in the question. Thanks a lot.

31 August 2020 5,347 3 View

How can I get H-bond versus time graph and hydrophobic residue distance versus time graph from Gromacs ?

I am using Gromacs to analyze the interactions between protein-ligand.I am a beginner of Gromacs simulation. I have finished the simulation, I got the final gro , xtc, cpt, edr files, but not...

05 August 2020 3,401 3 View

What is the physical meaning of the cross term of the index performance of LQR ?

Dear All : I have a curious question. Among the index performance of the LQR, X' Q X and u' R u have the physical meaning. Now, my question what is the physical meaning of the cross term X' S...

02 August 2020 1,780 1 View

How to make parrallel inlet boundary UDF work?

Hi everyone, It is quite urgent for me! I am doing 2D rotating detonation engine (RDE) simulation in ANSYS Fluent. For inlet boundary condition, there are three possible cases: 1. Pi > Po,...

02 August 2020 8,443 1 View

Extracting surface area, volume and cortical thickness from multiple groups using FreeSurfer?

Hello everyone, I'm new to FreeSurfer and this question might sound a bit stupid: I'm trying to get the volumetric measures, surface area and cortical thickness of brain regions of three groups...

29 July 2020 8,574 3 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

Weber Lin

Hi Jie Yin,

Which technique you performed? qPCR, WB...??

But no matter which technique you choose, you should normalize the expression level of your interested genes with reference gene or protein before you comparing the expression level between them.

Best,

Katie A Burnette

Yes, it is best to use one set of primers per plate with all of your samples. You will have to include a housekeeping control gene, a standard curve for your gene of interest, and a standard curve for the housekeeping gene on every plate. Plus positive and negative controls for each set of PCR primers. Basically, most of each plate will be your controls and references and only a small portion will be from your samples. Good luck!