How to compare expression levels of different genes of the same tissue?

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Noor Idan Jarad

https://www.biostars.org/p/50332

https://www.biostars.org/p/135010

Weber Lin

Hi Jie Yin,

Which technique you performed? qPCR, WB...??

But no matter which technique you choose, you should normalize the expression level of your interested genes with reference gene or protein before you comparing the expression level between them.

Best,

Katie A Burnette

Yes, it is best to use one set of primers per plate with all of your samples. You will have to include a housekeeping control gene, a standard curve for your gene of interest, and a standard curve for the housekeeping gene on every plate. Plus positive and negative controls for each set of PCR primers. Basically, most of each plate will be your controls and references and only a small portion will be from your samples. Good luck!