How to immerse bricks in wax ( for coating ) ?

Do we immerse the bricks horizontally or vertically ? #bricks#waterproofingsurface#civil#engeenering#chemicalengineering#wax#parafinwax

28 February 2021 6,685 2 View

What online teaching new topics to address as a phd topic?

Greetings, I have just started my phd in the field of english language studies, Im currently interested in online teaching, e-learning but mostly english language pedagogy. what are the new...

16 November 2020 4,045 9 View

Hi There any one can help on the case study below?

04 October 2020 7,234 1 View

Best strategy to transfect large genes in mammalian cells?

I have an insert ( ORF 6,9kb long) that I want to transfect in a human colon cancer cell line. I initially wanted to do with lentivirus, since I could have a stable expression of my gene and...

22 April 2020 3,277 3 View

What to do with coincident features for Geographically weighted regression?

I am sorry if this is a simple question. I am trying to conduct a GWR for hedonic analysis of hotel room rates. For OLS I have conducted analysis including different room types (e.g. double,...

16 April 2020 7,062 3 View

Can a conjugated isotype antibody be used in cell culture function blocking antibody experiment?

I have a PE conjugated mouse k isotype antibody..I would like to use it as control and add to cell culture .I would like to know whether conjugate will interfere in cell culture...

22 January 2020 607 2 View

What should be a loading control in condition media for zymography?

I want to do a zymography in condition media from cancer cell line...I would like to know what should be a loading control for condition media specific for zymography? Whether loading control can...

13 November 2019 1,697 1 View

What is the best way to analyze interview question with scale answer from 1-5?? could I use likert even my research is qualitative?

I have a structured interview to test a hypothesis, one of the questions required an answer from 1 to 5 .. I don't know how to analyze this? any recommendations? @qulitative analysis @Structured...

21 February 2019 6,307 11 View

What to chose for publishing IJISRT or IJCSIS ???

i have to chose for publishing between two journals 1- IJISRT (International Journal of Innovative Science and Research Technology) ,ISSN No.: 2456-2165,SJIF Impact Factor : 5.15, with feeds...

12 January 2019 8,736 31 View

Anyone help me to real time data analysis?

we optimized the gene expression with different condition and different concentration of the sample and we obtained the ct value in the range of above 26-30. The ct value for Housekeeping gene...

06 January 2019 6,677 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

Alternatives to traditional cloning of insert into backbone (dsDNA)?

Hi, I am stuck with a cloning experiment: The insert & backbone(bb) share one compatible end at 3' end(NotI) and one incompatible end at 5' end (XbaI for insert; HindIII for backbone). So i...

16 February 2021 1,664 3 View

Can part of recombinant protein not be expressed?

Hello. I am currently trying to express a recombinant gene with Avitag at the N-terminal. I'm aiming at a final product that looks like this: Avitag-(target protein) The protein I use is already...

14 February 2021 2,039 4 View

Which tropical country is the most easy for obtaining research permit (for biological research)?

Dear all, High beurocracy hihly complicate field ecolocial and evolutionary research nowadays. Nearly all tropical countries (maybe all?) requires research permit for performitng research and in...

04 February 2021 3,672 6 View

Transformation into the Stbl3 chemo competent cells?

03 February 2021 1,111 5 View

What is the best way and technique for serial dilution of bactria to get accurate number of colony ?

Hello I am infecting cells with bacteria in a well and then I am doing serial dilution of this well 1 in 10 and culture the two last dilution on MHA agar I have difficulty in getting accurate...

26 January 2021 9,256 5 View

Good day, I have not been able to amplify the plasmid Frt-hspB1. Somebody could give an advice me?

I acquired the plasmid in Addgene, reconstituted the filter paper where the plasmid comes from with 30 microliters of TE buffer pH 8, immediately afterwards perform the transformation of E.coli...

25 January 2021 2,526 3 View

Has someone used TTC agar overlay method and can help me?

I have tried to use TTC agar overlay method for a few times to identify respiratory-deficient yeast which were affected by EthBr. The problem is that cells which should have stayed white also have...

22 January 2021 585 10 View

How to distinguish pure and adulterated honey using NMR spectroscopy?

What are the common adulterants in commercial honey? how to identify pure honey and adulterated honey with the help of NMR spectroscopy? Is there any other technique to check purity of honey?

20 January 2021 5,456 12 View