Since hippocampal and microglial two cell lines are maintained in different temperature, I was wondering how to conduct the experiment having coculture between these two.
Cell Cytotoxicity
24 June 2024 7,671 2 View
How to measure STEM education What are the indicators? Where may I get the tool to assess STEM education? How can I check the impact of STEM education? I need a questionnaire, including questions...
04 November 2023 1,164 0 View
in order to create GelMA and Carboxymethyl Chitosan Hydrogel Scaffolds? Do I need to use EDC/NHS for crosslinking ? OR Lithium acylphosphinate photo-initiator (LAP) is enough as the crosslinking...
06 February 2023 5,543 1 View
do i need ethanol or just distil water ?what is the proper concentration to dissolve ?
27 December 2022 3,202 7 View
Journals related to Soil-structural interaction...
21 August 2022 7,116 4 View
Reference for this tool is The ostomy leak impact tool: Development and validation of a...
03 July 2022 772 0 View
The functions of borehole and tunnels are not working properly in Plaxis 2d v20 update 2.
09 March 2021 2,679 1 View
Codon optimization is not an option here because I need that specific template.
09 September 2020 1,247 0 View
This is certainly not a new question. This topic had to be researched after the Spanish Flu pandemic. Could you recommend any factual papers with actual numbers? Thank you
09 April 2020 8,684 0 View
I did some experiments infecting Salmonella Typhi with the lytic phage I isolated from the environment. It gives a nice clear plaques on agar overlay. However, in tube co-culture growth assay, the...
08 November 2019 5,035 5 View
What are the emerging trends and opportunities for STEM education and careers in the online space?
10 August 2024 507 0 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I'm currently working on a project where I need to plot the atom-projected band structure using GPAW. I've been able to calculate the band structure for my material, but I'm having trouble...
07 August 2024 269 3 View
Hi all, my experimental XPS results shown that my C3N5 sample consists of N-H bond, hence in this case I should incorporate the N-H bond into my DFT modelling. However, I do notice several papers...
07 August 2024 8,414 2 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View