How to calculate the adsorption capacity of material when the removal is 100% and UV vis reading is either zero or negative?

Hi, I am doing experiments with biochar for Cr(VI) and at 10 and 20 ppm concentration i am getting negative reading or zero reading with UV vis spectrometer. Also, i am using DPC method and no...

14 March 2019 5,313 11 View

Can anybody tell if there any upcoming conferences on Civil, Geotechnical or Dam Engineering?

I have been looking for conferences for a while now and I started reading about predatory conferences. Now I am not sure which conferences are genuinely legitimate? There are not many sites that...

01 November 2018 2,037 6 View

How to deplete MDSCs in mice bearing 4T1 tumors?

07 September 2016 8,375 4 View

At the time of El Nino, the trade winds are weaker at the equator region over the Pacific ocean why?

At the time of El Nino, the trade winds are weaker at the equator over the Pacific ocean why? please explain those who knows exact answer......

22 August 2016 6,794 8 View

How can I stably transfect 4T1 with gene of interest?

30 November 2015 4,941 4 View

Are there any techniques, other than ELISA, for measuring secreted protein (21KDa) from the conditioned media?

Since ELISA kits are very expensive, was wondering if I could measure that with western blot technique? and If yes, how to measure the protein concentration? For the cell lysate I use protease...

06 April 2015 2,460 7 View

What are the female hormones that can be detected by using HPLC-Flourescence detector?

What are the female hormones that can be detected by using HPLC-Flourescence detector?

29 January 2014 556 3 View

Why is interference seen in blank plasma?

Liquid-liquid extraction technique is used to extract the drug from plasma. No interference was observed in plasma which was purchased from the hospital. When the pharmacokinetic study was done in...

29 January 2014 1,463 4 View

BrdU or Nestin: which one is better to use as marker for proliferation assay?

Since both markers can be used to check the proliferation of neural stem cells, I was wondering what is the difference in using them? Is there a difference in the method using it or price? Can...

23 September 2013 5,826 7 View

The retention time of basic compound(pKa=9.5) is changing in every experiment. Can anyone suggest the solution?

Mobile phase: Acetonitrile:phosphate Buffer(20:80), pH 7, 0.1% triethylamine added Column : 4.6*150 mm c18 (end capped), Guard column (not more than 50 injections upto now) attached Temperature =...

25 April 2013 3,221 9 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View