We have anti-Trissacharide antibodies for detection through ELISA. We need to coat trissacharide on ELISA Plates. We were thinking about conjugating trissacharide to BSA via a EDA ethylenediamine linker. We can also use other linkers but we are not getting any protocol for this from literature.
Saleha Hafeez
With BSA you might have a huge background plus BSA as a protein with capacitytobind various molecules could give you a background.
Can you modify your trisacharide to introduce amine or thiol group? Alternativelly instead ob BSA, you could attach a biotin with a suitable linker.
Then if your ELISA plates NHS, malemide or streptavidin coated ,you can get a better immobilization with accesabiloity to antibodies for detection
Povilas Kavaliauskas
We have one aldehyde group in Gal mono of trisaccharide for reductive amination.
Saleha Hafeez with that if you have amine you can either use it for biotinilation and coat plates with streptavidin or simply coat the plates and crosslink it with glutaraldehyde.
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