Is there any open-source tool to cluster ca. 100 ITS sequences into OTUs using Windows? It seems that most of the softwares work on Mac or Linux...Also do you know if there is any online free server to do the clustering?
These are all free for Windows...
For cluster analysis you may prefer Taxon DNA's species identifier (download: http://taxondna.sourceforge.net/downloads.shtml), BEAST for GMYC,
Online tool for cluster analysis: ABGD, bPTP (need rooted/ ultrametric tree), Blast Clust., BIN (Barcode index number @ BOLD systems).
You can use MEGA for multiple alignment and also molecular taxonomic analysis. It is free to download (www.megasoftware.net/).
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4274427/
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