It's a 2.3kb fragment. Is it possible to amplify this gene from cDNA or cDNA synthesized from RNA of cocl2 treated cells ?
Hi Ayushi. Could you please explain me what is your exact problem with Hif1a cloning? What do u mean by fussion plymerase? Do you want subcloning for reporter activity study in you cell culture system?
What low-cut filter (high Pass frequency) to be used for the response spectrum (Tripartite plots)? I am currently working on differentiating between hard/soft site responses in Indo-Gangetic...
21 February 2021 9,273 2 View
I am going to prepare activated carbon from the waste activated sludge coming out of WWTP. Can you please help me in understanding, which chemical/physical activating agent will be most...
07 February 2021 9,507 5 View
I measured the UV-vis and EPR of the same polymer. In EPR there is a high intenisty polaron peak but in UV-vis spectroscopy the polaron peak is not visible.
31 May 2020 9,695 3 View
Which solvent system should be used for the monitoring of biscoumarin synthesis for TLC. as it is dissolved in DCM for spotting and when it is runned in 6: 4 ratio of ethylacetate or hexane but it...
31 August 2019 6,486 3 View
The tests have a number of subtests (eg Malin's Intelligence Scale for Indian Children, which has 11 subtests) and various parameters ( eg Wisconsin Card Sorting Tests , has a number of parameters...
02 November 2018 8,356 2 View
Need it for simulation purposes.
01 April 2018 6,680 2 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...
03 March 2021 401 4 View
Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...
01 March 2021 210 1 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...
28 February 2021 5,440 3 View
The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...
28 February 2021 6,560 14 View
Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?
28 February 2021 8,095 1 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View