Malay Chaklader

2 Questions 8 Answers 0 Followers

Questions related from Malay Chaklader

Did you guys experience cell death due to RPMI and CDM3 supplementation during differentiation of iPSc to cardiomyocyte?

I am trying to differentiate isogenic pairs of iPSC (derived from Trisomy 21 patients) in cardiomyocytes for the last couple of months but failed. First, I thought it could be the toxicity of the...

14 February 2021 3,026 12 View

How do you increased the yield of isolated crypts from small intestine and colon?

I have tried PBS-EDTA-DTT chelation based method for isolating intestinal crypts. However, I did not achieve high yield of crypts. Can any one help me to figure out this problem?

07 September 2014 506 1 View