How to choose an ATCC strain as control?

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What is the proper method for cleaning of combs for agarose gel electrophoresis?

Can we wash it by boiling it in water if the visible stains of ethidium bromide are not washed after rinsing with water and then 70% ethanol?

07 August 2018 8,943 3 View

Which software is better for Statistical Analysis, R or SPSS?

I need to analyze my data statistically, and I need a user friendly software with international recognition for publication, please suggest any software for that purpose. It would be great help if...

06 July 2018 3,888 12 View

What does number written between sequence of nucleotide means?

If there is a number superscript on the sequence of a primer what does it means? e.g. T153AGCT etc

11 December 2017 10,081 1 View

Can anyone recommend me any tools or software's for Viral In silico PCR and predicting DNA bands?

I have seen following...

11 December 2017 1,280 4 View

Does anyone know what is Global Impact Factor (GIF)?

should we publish in Journals having GIF, as they are charging very few amount of fee.

10 November 2017 7,949 0 View

Does anyone know about Academic Research International Journal?

Is this journal recognized or not? it is showing that it is indexed in Index Copernicus.

10 November 2017 4,813 6 View

What is the best method for Enhancing Gene Expression?

What is best method for enhancing the expression of a gene if you need a protein, is it increasing copy no or removing control from it or anything else?

10 November 2017 7,366 5 View

How quickly the PCR primer deteriorates?

I used to use PCR primers even after 3-4 months and they worked, but now something seems to be wrong with them, they stop working after few runs. I am totally clueless about it.

09 October 2017 1,507 6 View

What is the difference between normal primers and primers for cloning?

I need to work on expression of a gene. can anyone tell me about the difference of these primers?

09 October 2017 6,608 4 View

Can we use any reverse transcriptase enzyme with any type of buffer?

when we use reverse transcriptase do we have to use specific buffer with that or any taq buffer can also work with that?

09 October 2017 1,745 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

How is the bacterial genome's high protein count verified as genuine despite 800+ contigs and good metrics (98.55%completeness, 0.68% contamination)?

Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...

01 August 2024 1,514 1 View

Is it common for heterotrophic bacteria to grow on Pro99 and SNAX media?

I am attempting to isolate picocyanobacteria from seawater (pre-filtered with a GF/D membrane) using the pour plate technique and filter plating method (Kearney et al., 2022) on Pro99 and SNAX...

30 July 2024 1,040 1 View

Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?

Hi, researchers, Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?

29 July 2024 4,570 0 View

Recovery Viurses from bacteria genome?

Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...

28 July 2024 8,953 1 View