We have tried traditional Protienase K method but we have not getting chloroplast DNA.
So anyone give some advice to proceed my work
The stress-strain calculations, energy density GI index calculations are also being done.
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The Smart Site Safety System (SSSS) by viAct is a one stop solution to secure a manufacturing facility using AI powered safety monitoring system. The ways in which Smart Site Safety System (SSSS)...
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Am a researcher working on Routing Protocols for underwater sensor networks. Can someone please share the MATLAB source codes of an AUV assisted routing protocol....Help me please... Thank u in...
12 November 2023 1,636 1 View
Where can I find the stress-strain relation in terms of Young's modulus, Poission's ratio, and components of contravariant metric tensor?
08 August 2023 363 2 View
Hi Every one I recetly bought desktop with speed of 3.60Ghz. While installing Genemapper v5 it says " The prcess is less than the required speed of 2.0Ghz. This setup will be aborted. I shall...
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In the powder metallurgy process, what is the sintering temperature and time required for AZ31 samples to get good mechanical properties?
21 May 2023 7,568 3 View
Originality of research
10 May 2023 9,305 3 View
Am a researcher working on Efficient Routing Protocols for underwater sensor networks. Am using MATLAB tool. Can someone help me with the detailed procedure of writing an environment, reward...
11 April 2023 4,557 2 View
viAct Smart Site Safety System use a combination of AI video analytics, cameras, and artificial intelligence (AI) to detect PPE in the workplace. AI Video Analytics: Smart Site Safety Systems use...
09 March 2023 6,151 1 View
Where and how could I get atmospheric CO2 concentration (not emission) grided data for Europe? I am looking for a high-resolution (not lower than 0.01°) spatiotemporal dataset for 2015-2021.
05 March 2023 5,310 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
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I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
It has been documented that apoptotic cells themselves can induce phosphorylation of serine 139 on H2AX (γH2AX) due to DNA fragmentation during apoptosis (doi: 10.1074/jbc.275.13.9390). As γH2AX...
28 July 2024 7,983 2 View
Hi, Now I am doing a single-base editing by using HiFi Cas 9, guideRNA, single stranded DNA, HDR Enhancer V2 and my tranfection mehtod is lipofection (RNAiMax). Unfortunately, after gene editing,...
26 July 2024 2,136 0 View
Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...
25 July 2024 4,927 3 View
Using INTRON's DNA-spin Plasmid DNA purification kit to extract plasmids from specific E.Coli strains, my team and I have encountered numerous plasmid dimerizations that lead to over-weighted...
23 July 2024 3,490 6 View