Dear Researchers,
I had deposited amino acids on Si-wafers by sublimation at different temperatures. Now I want to know whether the layer contains monomers, polymers or fragmentation products. I found special ATR-IR spectroscopes to handle with wafers in literature, but I only have access to standard instrumentation for liquids and powders. If there is no way to check the structure directly on the wafers, I will dissolve the layers after finishing all characterisations and use standard NMR-, IR- and MS-techniques, but first I will look for the wafer layer conserving techniques. Perhaps somebody knows about an institution or research group in the region of Hamburg, Germany, with option for measuring with above-mentioned wafer holder containing ATR-IR spectroscopes. Please, let me know!
I would suggest using transmission mode to record spectra of the deposits on these wafers. Use an uncoated silicon wafer as a background reference. You can just place the wafer in the sample compartment of your spectrometer.
Michael, I think there could be no advice better than yours !
Leonid
Please try, Sebastian !
I myself almost always use Si-wafers in place of KBr or other optical Salt-monocrystals.
Si lattice vibrations - lay in low frequencies - so if the wafer is rather thin (
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Biology
- Biomolecules
- Nucleic Acids
- RNA