01 January 1970 1 5K Report

I made a kind of gene knock-in mice, the vector was inserted after the endogenous promoter of this gene. For the vector, it includes part of exons of WT gene and the WT fragment is flanked by two loxP sites following which there is a part of exons containing amino acid mutant(one amino acid is mutated to another). So this gene-KI F/F mouse expresses WT gene and it expresses gene mutant in which case it breeds with Cre mice. Is there any method to detect the gene expression of this mutant? Western blot cannot work because the length of this gene is consistent......

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